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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2369R |
| 英文名称 | Cytokeratin 3+12 Rabbit pAb |
| 中文名称 | 细胞角蛋白3+12抗体 |
| 英文别名 | CK 12; CK 3; CK12; CK3; Cytokeratin 12; Cytokeratin 3; K12; K3; keratin 12(Meesmann corneal dystrophy); Keratin 12; Keratin 3; Keratin, type I cytoskeletal 12; Keratin, type II cytoskeletal 3; KRT12; KRT3; 65 kDa cytokeratin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cytokeratin 3+12 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 65+54 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Immune System Diseases > Autoimmune Neuroscience > Sensory System > Visual system Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Intermediate Filaments > Class I > Cytokeratins |
| 亚基 | Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12. |
| 组织特异性 | Cornea specific. |
| 相似性 | Belongs to the intermediate filament family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 3.4 }}] {Haiqiong Li. et al. MUC1‑ND interacts with TRPV1 to promote corneal epithelial cell proliferation in diabetic dry eye mice by partly activating the AKT signaling pathway. MOL MED REP. 2024 Dec;30(6):1-14} {IF} {Mouse}
[IF={{ 3.231 }}] {Xu W et al. An in situ hydrogel based on carboxymethyl chitosan and sodium alginate dialdehyde for corneal wound healing after alkali burn.(2018) J Biomed Mater Res A.} {IF} {Rabbit}
Immuno-Laser Capture Microdissection
in heterogeneous tissues has been significantly expanded. Advantages of Immuno-LCM Sections fixed in acetone, methanol, or ethanol/acetone give excellent immunostaining after 12 to 25 minutes total
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
Studying Wound Repair in the Mouse
:1.8.1‐1.8.4. Galiano, R.D., Michaels, J., Dobryansky, M., Levine, J.P. and Gurtner, G.C. 2004. Quantitative and reproducible murine model of excisional wound healing. Wound Repair Regen. 12:485‐492
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