SGK1 | SGK (p-S78) Rabbit pAb(bs-3395R)-50ul/100ul/200ul产品图
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SGK1 | SGK (p-S78) Rabbit pAb(

bs-3395R)-50ul/100ul/200ul
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  • 2026年10月14日
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    北京博奥森生物技术有限公司
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    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3395R
    英文名称phospho-SGK1 (Ser78) Rabbit pAb
    中文名称磷酸化糖皮质激素调节激酶1抗体
    英文别名SGK1(phospho S78); p-SGK1(phospho S78); Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serine/threonine-protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK 1; SGK; SGK1; SGK1_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human SGK1 around the phosphorylation site of Ser78
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量49 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Developmental Biology > Embryogenesis > Embryonic stem cells > Surface molecules

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases

    Stem Cells > Embryonic Stem Cells > Intracellular

    亚基Interacts with NEDD4 and NEDD4L.
    亚细胞定位Isoform 2: Cell membrane.
    Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation.
    组织特异性Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
    翻译后修饰Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
    Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
    相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    功能Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料SGK1 is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels of SGK1 and activity may contribute to conditions such as hypertension and diabetic nephropathy. This protein also mediates cell survival signals, as it has been shown to phosphorylate and negatively regulate the pro apoptotic FOXO3A protein. Ser 422 is a critical site on the protein and may be involved in its activation.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: human kidney tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-Phospho-SGK1(Ser78) Polyclonal Antibody, Unconjugated(bs-3395R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_ _x000D_
    产品细节图片2
    Sample: A431 lysate at 30 ug
    _x000D_ Primary: Rabbit Anti-Phospho-SGK1 (bs-3395R) at 1:300
    _x000D_ Secondary:IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size:49 kD
    _x000D_ Observed band size:49 kD
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-SGK1 (Ser78)) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_ _x000D_
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-SGK1 (Ser78)) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_ _x000D_
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-SGK1 (Ser78)) Polyclonal Antibody, Unconjugated (bs-3395R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_ _x000D_
    产品细节图片6
    Sample:
    _x000D_ Pancreas (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-Phospho-SGK1 (Ser78) (bs-3395R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 49 kD
    _x000D_ Observed band size: 50 kD
    产品细节图片7
    Sample:
    _x000D_ Lane 1: Mouse Cerebrum tissue lysates
    _x000D_ Lane 2: Rat Cerebrum tissue lysates
    _x000D_ Primary: Anti-Phospho-SGK1 (Ser78) (bs-3395R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 49 kDa
    _x000D_ Observed band size: 49 kDa

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    该产品被引用文献

    [IF=1.7] Kumiko Taguchi. et al. Esaxerenone Improves Vascular Endothelial Dysfunction by Reducing Serum and Glucocorticoid-Regulated Kinase 1 Activity and Enhancing the Akt Pathway in Type 2 Diabetic Mice. BIOL PHARM BULL. 2025 Apr;48(4):422-431 WB Mouse

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    SGK1 | SGK (p-S78) Rabbit pAb(bs-3395R)-50ul/100ul/200ul
    ￥1180 - 2800