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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原:
recombinant protein
- 形态:
liquid
- 保存条件:
Store at -20˚C
- 克隆性:
Monoclonal antibody
- 适应物种:
Human;Mouse;Rat
- 保质期:
12 months
- 抗原来源:
Recombinant Rabbit
- 供应商:
南京赛戈巍生物科技有限公司
- 宿主:
Recombinant Rabbit
- 应用范围:
WB, IHC, IP, FC, IF
- 靶点:
Uniprot:O00141
- 抗体英文名:
SGK1 Rabbit mAb
- 规格:
50ul/100ul
Serine/threonine protein kinase SGK antibody
Serine/threonine protein kinase Sgk1 antibody
Serine/threonine-protein kinase Sgk1 antibody
Serum and glucocorticoid regulated kinase antibody
Serum/glucocorticoid regulated kinase 1 antibody
Serum/glucocorticoid regulated kinase antibody
Serum/glucocorticoid-regulated kinase 1 antibody
SGK 1 antibody
SGK antibody
SGK1 antibody
Sgk1 variant i3 antibody
SGK1_HUMAN antibody
计算分子量:55 kDa
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:1,000
IHC:1:50-1:200
IF: 1:200
FC: 1:50-1:100
图片:

Western blot analysis of SGK1 on human kidney lysates using anti-SGK1 antibody at 1/1,000 dilution.
,

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
,

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
,

Flow cytometric analysis of 293 cells with SGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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- 作者
- 内容
- 询问日期
文献和实验:使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。(图 A)使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit,抗体 1:100 稀释;(图 B) 采用普通免疫组化试剂盒,抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注:使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK (Erk1/2)Rabbit mAb 对正常小鼠心脏组织进行免疫
h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
到 PVDF 膜上,同时减少蛋白不必要降解,这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息:1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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