Blank control(blue): RAJI(fixed with 2% Paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-Phospho-PLC beta3 (Ser1105) antibody(bs-3341R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
- ￥1180 - 2800
- Bioss
- bs-3341R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
phospho-14-3-3 beta + zeta (Ser186 / Ser184) Rabbit pAb, BF647 conjugated(bs-13773R-BF647)-100ul
￥2980EpCAM Rabbit pAb, Biotin conjugated(bs-4889R-Bio)-100ul
￥2980
CTDSPL Rabbit pAb(bs-8241R)-50ul/100ul/200ul
￥1180
HDAC3 Mouse mAb(bsm-60265M)-50ul/100ul
￥1580SULF1 Rabbit pAb, BF680 conjugated(bs-5818R-BF680)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3341R |
| 英文名称 | phospho-PLCB3 (Ser1105) Rabbit pAb |
| 中文名称 | 磷酸化磷酯酶Cβ3抗体 |
| 英文别名 | PLCB3_HUMAN; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-3; 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-3; EC:3.1.4.11; phosphoinositide phospholipase C-beta-3; phospholipase C-beta-3(PLC-beta-3); SMDCD; phospholipase C beta 3; |
| 产品应用 | ICC/IF=1:100-500, Flow-Cyt=1μg /Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human PLC beta3 around the phosphorylation site of Ser1105 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 136 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Lipid metabolism Signal Transduction > Signaling Pathway > Lipid Signaling > PLC |
| 亚基 | Interacts with SHANK2. Interacts with LPAR2. |
| 亚细胞定位 | Membrane; Peripheral membrane protein. |
| 相似性 | Contains 1 C2 domain. Contains 1 PI-PLC X-box domain. Contains 1 PI-PLC Y-box domain. |
| 功能 | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. |
| 应用 | 推荐稀释比例 |
| {ICC/IF} | {1:100-500} |
| {Flow-Cyt} | {1μg /Test} |
MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PLC beta3 (Ser1105) ) polyclonal Antibody, Unconjugated (bs-3341R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 16.6 }}] {Ding Yangyang. et al. MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis. NAT COMMUN. 2023 Aug;14(1):1-15} {WB} {Mouse}
相关实验
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
技术资料暂无技术资料 索取技术资料
文献支持
phospho-PLCB3 (Ser1105) Rabbit pAb(bs-3341R)-50ul/100ul/200ul
￥1180 - 2800
