Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-GRF2 (bs-16311R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kD
Observed band size: 120 kD
- ￥1180 - 2800
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- bs-16311R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-16311R |
| 英文名称 | GRF2 Rabbit pAb |
| 中文名称 | G蛋白偶联受体释放因子2抗体 |
| 英文别名 | C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2(specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor(GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GRF2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 140 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Ras Family Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Regulators |
| 组织特异性 | Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver. |
| 相似性 | Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. |
| 功能 | Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HL-60 cell lysates
Lane 3: Human JAR cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-GRF2 (bs-16311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa
Lane 1: Human MCF-7 cell lysates
Lane 2: Human HL-60 cell lysates
Lane 3: Human JAR cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-GRF2 (bs-16311R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa
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GRF2 Rabbit pAb(bs-16311R)-50ul/100ul/200ul
￥1180 - 2800
