EXO1 Rabbit pAb(bs-13119R)-50ul/100ul/200ul产品图
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EXO1 Rabbit pAb(bs-13119R)-50u

l/100ul/200ul
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  • ￥1180 - 2800
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  • bs-13119R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-13119R
    英文名称Exonuclease 1 Rabbit pAb
    中文名称核酸外切酶1抗体
    英文别名exo1; EXO1_HUMAN; ExoI; Exonuclease 1; Exonuclease I; Exonuclease1; HEX1; hExo I; hExo1; hExoI; Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangu)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Exonuclease 1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量94 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚基Interacts with the MLH1-PMS2 heterodimer via MLH1. Interacts with MSH3. Interacts with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. Interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. Interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5'-overhanging flap structures. Interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5'-overhanging flap structures.
    亚细胞定位Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
    组织特异性Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
    翻译后修饰Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
    相似性Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
    功能5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the Exo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/EXO1 gene to chromosome 1q43. Exo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The MMR system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MMR. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1ug/Test}

     

    产品细节图片1
    Blank control (Black line):Molt4 (Black).
    _x000D_ Primary Antibody (green line): Rabbit Anti-Exonuclease 1 antibody (bs-13119R)
    _x000D_ Dilution: 1μg /10^6 cells;
    _x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
    _x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
    _x000D_ Dilution: 1μg /test.
    _x000D_ Protocol
    _x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Exonuclease 1) Polyclonal Antibody, Unconjugated (bs-13119R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片3
    Sample:
    _x000D_ Testis (Mouse) Lysate at 40 ug
    _x000D_ Thymus (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti-Exonuclease 1 (bs-13119R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 94 kD
    _x000D_ Observed band size: 90 kD
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Exonuclease 1 Polyclonal Antibody, Unconjugated (bs-13119R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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    该产品被引用文献

    [IF=3.8] Li, Zhuoqi. et al.EXO1 is a key gene for lung-resident memory T cells and has diagnostic and predictive values for lung adenocarcinoma.SCIENTIFIC REPORTS.2025 Feb 1;15(1):4002. Rabbit

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    EXO1 Rabbit pAb(bs-13119R)-50ul/100ul/200ul
    ￥1180 - 2800