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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-11968R |
| 英文名称 | Semaphorin 3D Rabbit pAb |
| 中文名称 | 跨膜蛋白SEMA3D抗体 |
| 英文别名 | Coll 2; Coll2; Collapsin 2; SEMA3D; Semaphorin3D; SEM3D_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Semaphorin 3D |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 85 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurology process > Growth and Development > Axonal Guidance Proteins Neuroscience > Neurology process > Neurogenesis Neuroscience > Neurology process > Neuroregeneration > Neuroregeneration |
| 亚细胞定位 | Secreted |
| 相似性 | Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain. |
| 功能 | One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. The class 3 semaphorins belong to the secreted group. Via interaction with its neuropilin receptor, semaphorin 3D plays a specific role in the induction of paralysis and collapse of neuronal growth cones. Semaphorin 3D could potentially act as repulsive cues toward specific neuronal populations. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA3D (semaphorin-3D), also known as coll-2 or Sema-Z2, is a 777 amino acid secreted protein that belongs to the semaphorin family and participates in axon-axon interactions and neural crest activity. SEMA3D binds neuropilin and is able to induce the collapse and paralysis of neuronal growth cones, as well as act to repel specific neuronal populations. SEMA3D contains one immunoglobulin-like (Ig-like) domain, one PSI domain and one semaphorin domain. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Semaphorin 3D) Polyclonal Antibody, Unconjugated (bs-11968R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Semaphorin 3D) Polyclonal Antibody, Unconjugated (bs-11968R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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Semaphorin 3D Rabbit pAb(bs-11968R)-50ul/100ul/200ul
¥1180 - 2800





