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Semaphorin 3D Rabbit pAb(bs-11

968R)-50ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-11968R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-11968R
    英文名称Semaphorin 3D Rabbit pAb
    中文名称跨膜蛋白SEMA3D抗体
    英文别名Coll 2; Coll2; Collapsin 2; SEMA3D; Semaphorin3D; SEM3D_HUMAN.
    产品应用IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human, Chicken, Dog, Pig, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Semaphorin 3D
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量85 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Neurology process > Growth and Development > Axonal Guidance Proteins

    Neuroscience > Neurology process > Neurogenesis

    Neuroscience > Neurology process > Neuroregeneration > Neuroregeneration

    亚细胞定位Secreted
    相似性Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
    功能One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. The class 3 semaphorins belong to the secreted group. Via interaction with its neuropilin receptor, semaphorin 3D plays a specific role in the induction of paralysis and collapse of neuronal growth cones. Semaphorin 3D could potentially act as repulsive cues toward specific neuronal populations.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA3D (semaphorin-3D), also known as coll-2 or Sema-Z2, is a 777 amino acid secreted protein that belongs to the semaphorin family and participates in axon-axon interactions and neural crest activity. SEMA3D binds neuropilin and is able to induce the collapse and paralysis of neuronal growth cones, as well as act to repel specific neuronal populations. SEMA3D contains one immunoglobulin-like (Ig-like) domain, one PSI domain and one semaphorin domain.

     

    应用推荐稀释比例
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Semaphorin 3D) Polyclonal Antibody, Unconjugated (bs-11968R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Semaphorin 3D) Polyclonal Antibody, Unconjugated (bs-11968R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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    [IF={{ 3.26 }}] {Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).} {IHC} {="Chicken"}

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    Semaphorin 3D Rabbit pAb(bs-11968R)-50ul/100ul/200ul
    ¥1180 - 2800