CALU Rabbit pAb(bs-6658R)-50ul/100ul/200ul

CALU Rabbit pAb(bs-6658R)-50ul

/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-6658R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-6658R
    英文名称CALU Rabbit pAb
    中文名称钙腔蛋白抗体
    英文别名CALU_HUMAN; Calumenin; Crocalbin; crocalbin-like protein; multiple EF-hand protein; IEF SSP 9302.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Calumenin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量35 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚基Interacts with GGCX.
    亚细胞定位Endoplasmic reticulum lumen. Secreted. Melanosome. Sarcoplasmic reticulum lumen. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    组织特异性Ubiquitously expressed. Expressed at high levels in heart, placenta and skeletal muscle, at lower levels in lung, kidney and pancreas and at very low levels in brain and liver.
    相似性Belongs to the CREC family.
    Contains 6 EF-hand domains.
    功能Involved in regulation of vitamin K-dependent carboxylation of multiple amino-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    CALU Rabbit pAb(bs-6658R)-50ul
    Sample:
    Muscle (Mouse) Lysate at 40 ug
    Primary: Anti-Calumenin (bs-6658R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 35 kD
    Observed band size: 40 kD
    CALU Rabbit pAb(bs-6658R)-50ul
    Tissue/cell: rat brain tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Calumenin Polyclonal Antibody, Unconjugated(bs-6658R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    CALU Rabbit pAb(bs-6658R)-50ul
    Tissue/cell: human cervical carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Calumenin Polyclonal Antibody, Unconjugated(bs-6658R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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