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ACPT Rabbit pAb(bs-6289R)-50ul

/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-6289R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    • 规格

      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-6289R
    英文名称ACPT Rabbit pAb
    中文名称睾丸酸性磷酸酶抗体
    英文别名Acid phosphatase testicular; Testicular acid phosphatase; PPAT_HUMAN.
    产品应用Flow-Cyt=0.2ug/test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human (Mouse, Rat, Dog, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human ACPT
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量47 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚细胞定位Membrane; Single-pass type II membrane protein (Potential).
    组织特异性High levels in pancreatic, gastric, colorectal and ampullary cancer. Very weak expression in normal gastrointestinal and urogenital tract.
    相似性Belongs to the peptidase S1 family.
    Contains 1 LDL-receptor class A domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Acid phosphatases are enzymes capable of hydrolyzing orthophosphoric acid esters in an acid medium. This gene is upregulated by androgens and is down-regulated by estrogens in the prostate cancer cell line. ACPT dephosphorylates receptor tyrosine-protein kinase erbB-4 and inhibits the ligand-induced proteolytic cleavage. ACPT is predominantly expressed in testis but exhibits a lower level of expression in testicular cancer tissues than in normal tissues. ACPT has structural similarity to prostatic and lysosomal acid phosphatases. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

     

    应用推荐稀释比例
    {Flow-Cyt}{0.2ug/test}

     

    产品细节图片1
    Blank control: Hela.
    Primary Antibody (green line): Rabbit Anti-ACPT antibody (bs-6289R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 0.2μg /test.
    Protocol
    The cells were incubated in 5 %BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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    图标文献和实验
    相关实验
    • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine

      = NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific

    • 亲和层析凝胶指南

      GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin

    • 亲和层析凝胶指南

      GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin

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    ACPT Rabbit pAb(bs-6289R)-50ul/100ul/200ul
    ¥1180 - 2800