Tissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(bs-2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-2684R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2684R |
| 英文名称 | GPNMB Rabbit pAb |
| 中文名称 | GPNMB抗体 |
| 英文别名 | NMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL; Dchil; ipd; Glycoprotein(transmembrane) nmb; Glycoprotein nmb; HGFIN; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB; Osteoactivin; GPNMB_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Pig, Cow, Horse, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPNMB |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 61 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > Tumor-associated antigens Immunology > Innate Immunity > Macrophage / Inflamm. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 组织特异性 | Not restricted to the melanocytic lineage. |
| 相似性 | Belongs to the PMEL/NMB family.
Contains 1 PKD domain. |
| 功能 | Could be a melanogenic enzyme. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Recombinant human GPNMB protein, C-His (HEK293)(bs-43555P)
Primary: Anti-GPNMB (bs-2684R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa
Lane 1: Recombinant human GPNMB protein, C-His (HEK293)(bs-43555P)
Primary: Anti-GPNMB (bs-2684R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 65 kDa
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文献和实验该产品被引用文献
[IF={{ 7.2 }}] {Aubrey Converse. et al. Multinucleated giant cells are hallmarks of ovarian aging with unique immune and degradation-associated molecular signatures. PLOS BIOL. 2025 Jun;23(6):e3003204} {IHC} {Mouse}
[IF={{ 6.847 }}] {Carmen Härdtner. et al. A comparative gene expression matrix in Apoe-deficient mice identifies unique and atherosclerotic disease stage-specific gene regulation patterns in monocytes and macrophages. ATHEROSCLEROSIS. 2023 Apr;371:1} {IF} {Mouse}
[IF={{ 6.1 }}] {Zixuan Fang. et al.Single-cell transcriptomic analysis reveals characteristic feature of macrophage reprogramming in liver Mallory-Denk bodies pathogenesis.JOURNAL OF TRANSLATIONAL MEDICINE.2025 Jan 16;23(1):77.} {IF} {Mouse}
[IF={{ 5.17 }}] {Oyewumi et al. Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells. (2016) Oncotarge. 7:13932-44} {IHC} {Human}
[IF={{ 5.1 }}] {Neal et al. The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor. (2018) J.Neuroinflammation. 15:73} {ICC} {Mouse}
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GPNMB Rabbit pAb(bs-2684R)-50ul/100ul/200ul
￥1180 - 2800
