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Big dynorphin Rabbit pAb(bs-14

473R)-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-14473R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-14473R
    英文名称Big dynorphin Rabbit pAb
    中文名称强啡肽抗体
    英文别名Dynorphin B; beta-neoendorphin-dynorphin; Big dynorphin; Big Dyn; Dyn B; Dyn-B; Dynorphin B; Dynorphin B(1-13); Dynorphin B-29; Leumorphin; PDYN; PDYN_HUMAN; Preprodynorphin.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Big dynorphin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量26 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Neurotransmitter > Neuropeptides > Opioids

    亚细胞定位Secreted.
    翻译后修饰The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
    相似性Belongs to the opioid neuropeptide precursor family.
    功能Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin. Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including C‌‌ocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Big dynorphin) Polyclonal Antibody, Unconjugated (bs-14473R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片2
    Sample:
    Lane 1: Mouse Cerebrum tissue lysates
    Lane 2: Mouse Cerebellum tissue lysates
    Lane 3: Mouse Hippocampus tissue lysates
    Lane 4: Rat Cerebrum tissue lysates
    Lane 5: Rat Cerebellum tissue lysates
    Lane 6: Rat Hippocampus tissue lysates
    Lane 7: Human U87MG cell lysates
    Primary: Anti-Big dynorphin (bs-14473R) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Big dynorphin) Polyclonal Antibody, Unconjugated (bs-14473R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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