Blank control: RSC96(blue).
Primary Antibody:Rabbit Anti- DRD5 antibody(bs-1747R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% Paraformaldehyde (10 min) . Antibody (bs-1747R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1747R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-1747R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1747R |
| 英文名称 | Dopamine D5 receptor Rabbit pAb |
| 中文名称 | 多巴胺受体D5抗体 |
| 英文别名 | D(1B) dopamine receptor; D(5) dopamine receptor; D1 beta dopamine receptor; D1B dopamine receptor; D1beta dopamine receptor; D5 dopamine receptor; DBDR; Dopamine receptor D1B; DRD 1B; DRD 5; DRD1B; DRD1L2; DRD5; MGC10601; DRD5_HUMAN; DRD5_RAT. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from rat DRD5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 53 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Dopamine Receptors Neuroscience > Neurotransmitter > Biogenic Amines > Dopamine Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Neuron-specific, localized primarily within limbic regions of the brain. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. This receptor is neuron specific, localized primarily within limbic regions of the brain. Defects in DRD5 are a cause of blepharospasm and may be a cause of schizophrenia. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg /test} |
Sample:
Skin(Mouse) Lysate at 40 ug
Skin(Rat) Lysate at 40 ug
Primary: Anti-Dopamine D5 receptor (bs-1747R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Skin(Mouse) Lysate at 40 ug
Skin(Rat) Lysate at 40 ug
Primary: Anti-Dopamine D5 receptor (bs-1747R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D5 receptor (bs-1747R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53kD
Observed band size: 53kD
Brain (Mouse) Lysate at 40 ug
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Dopamine D5 receptor (bs-1747R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53kD
Observed band size: 53kD
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文献和实验该产品被引用文献
[IF={{ 8.101 }}] {Linlin Meng. et al. Dopamine D1 receptor agonist alleviates acute lung injury via modulating inflammatory responses in macrophages and barrier function in airway epithelial cells. FREE RADICAL BIO MED. 2023 Jun;202:2} {IHC} {Mouse}
[IF={{ 4.522 }}] {Fujino S et al. Expression and function of dopamine in odontoblasts. J Cell Physiol. 2019 Oct 15.} {IHC,WB} {Rat}
[IF={{ 4.181 }}] {Bai L et al. m6A demethylase FTO regulates dopaminergic neurotransmission deficits caused by arsenite.Toxicol Sci. 2018 Oct 1;165(2):431-446.} {WB} {Mouse}
[IF={{ 2.35 }}] {Xu, Jiao-jiao, et al. "Dopamine D1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells." Acta Pharmacologica Sinica (2014).} {WB} {="Human"}
[IF={{ 11.685 }}] {Kristine Elisabeth Eberhard. et al. Neurotransmitter and Neurotransmitter Receptor Expression in the Saccule of the Human Vestibular System. Prog Neurobiol. 2022 Jan;:102238} {IHC} {Human}
相关实验
Characterization of Dopamine Receptors
of the radioligand's molar concentration is also included. Keywords: Receptor binding; dopamine; D1; D2; D3; D4; D5; radioligand binding GO TO THE FULL PROTOCOL:
Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells
in these microdomains. Dopamine D1 -like receptors (D1 R and D5 R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed
In mammals, dopamine G protein-coupled receptors (GPCR) are segregated into two categories: D1-like (D1R and D5R) and D2-like (D2Rshort , D2Rlong , D3R, and D4R) subtypes. D1R and D5R are primarily coupled to stimulatory heterotrimeric GTP
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Dopamine D5 receptor Rabbit pAb(bs-1747R)-50ul/100ul/200ul
￥1180 - 2800
