Tissue/cell: colonic epithelial cells;
Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-REA Polyclonal Antibody, Unconjugated(bs-10283R) 1:100, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-10283R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10283R |
| 英文名称 | PHB2 Rabbit pAb |
| 中文名称 | B细胞受体相关蛋白抗体 |
| 英文别名 | PHB2_HUMAN; Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity; BAP; REA; p22; hBAP; Bap37; BCAP37; PNAS-141; |
| 产品应用 | WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PHB2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 33 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Estrogen Signal Transduction > Growth Factors/Hormones > Hormones Signal Transduction > Protein Trafficking > Chaperones > Other Chaperones Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Co-activators/co-repressors Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Estrogen |
| 亚基 | Interacts with PHB, ESR1, HDAC1 and HDAC5. Interacts with ZNF703. |
| 亚细胞定位 | Mitochondrion inner membrane. Cytoplasm. Nucleus. Also cytoplasmic and nuclear. |
| 相似性 | Belongs to the prohibitin family. |
| 功能 | Acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. Functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. Competes with NCOA1 for modulation of ER transcriptional activity. Probably involved in regulating mitochondrial respiration activity and in aging. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables several functions, including protein C-terminus binding activity; protein N-terminus binding activity; and protein dimerization activity. Involved in several processes, including defense response to virus; positive regulation of cell cycle phase transition; and regulation of transcription, DNA-templated. Located in several cellular components, including cell surface; mitochondrial membrane; and nuclear matrix. Part of mitochondrial prohibitin complex. [provided by Alliance of Genome Resources, Apr 2022] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Protein: spleen(mouse) lysate at 40ug;
Primary: rabbit Anti-REA (bs-10283R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 33 kD
Observed band size: 35 kD
Primary: rabbit Anti-REA (bs-10283R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 33 kD
Observed band size: 35 kD
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Rat Kidney tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human 293T cell lysates
Lane 8: Human HT-1080 cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human A549 cell lysates
Primary: Anti-PHB2 (bs-10283R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 35 kDa
Lane 1: Mouse Kidney tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Rat Kidney tissue lysates
Lane 5: Rat Testis tissue lysates
Lane 6: Human HeLa cell lysates
Lane 7: Human 293T cell lysates
Lane 8: Human HT-1080 cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human A549 cell lysates
Primary: Anti-PHB2 (bs-10283R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 35 kDa
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PHB2) polyclonal Antibody, Unconjugated (bs-10283R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-PHB2 antibody (bs-10283R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-PHB2 antibody (bs-10283R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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PHB2 Rabbit pAb(bs-10283R)-50ul/100ul/200ul
￥1180 - 2800
