Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FAM3B/PANDER Polyclonal Antibody, Unconjugated(bs-0711R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0711R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Ear5 Rabbit pAb(bs-10605R)-50ul/100ul/200ul
￥1180
PKC delta Rabbit pAb, BF555 conjugated(bs-23441R-BF555)-100ul
￥2980phospho-14-3-3 theta (Ser232) Rabbit pAb, PE-Cy7 conjugated(bs-13774R-PE-Cy7)-100ul
￥2980CD73 Rabbit pAb, APC-Cy5.5 conjugated(bs-4834R-APC-Cy5.5)-100ul
￥2980LHFPL1 Rabbit pAb, AP conjugated(bs-18239R-AP)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0711R |
| 英文名称 | FAM3B Rabbit pAb |
| 中文名称 | 胰腺衍生因子抗体 |
| 英文别名 | C21orf11; PANDER; Cytokine like protein 2 21; PRED44; PANcreatic DERived factor; Protein FAM3B; Protein FAM3B precursor; Family with sequence similarity 3, member B isoform a; FAM3B_MOUSE. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FAM3B |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Innate Immunity > Cytokines Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Metabolic disorders Metabolism > Types of disease > Obesity |
| 亚细胞定位 | Secreted. Note=Present in insulin secretory granules and likely cosecreted with insulin. Localized in discrete vesicular and perinuclear structure. |
| 组织特异性 | Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis. |
| 翻译后修饰 | 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46.
O-glycosylated. |
| 相似性 | Belongs to the FAM3 family. |
| 功能 | Induces apoptosis of alpha and beta cells in a dose- and time-dependent manner. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | FAM3B has delayed effects on beta-cell function, inhibiting basal insulin secretion from a beta-cell line in a dose-dependent manner. [Subcellular Location] Secreted. Note=Localized in discrete vesicular and perinuclear structure. Highly expressed in the pancreas. Also found in the colon, kidney, prostate, small intestine and testis. 2 N-termini have been observed in the mature protein: the first at Glu-30, resulting from signal peptide cleavage, the second at Ser-46. Belongs to the FAM3 family. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
技术资料暂无技术资料 索取技术资料
FAM3B Rabbit pAb(bs-0711R)-50ul/100ul/200ul
￥1180 - 2800
