Tissue/cell: Rat skeletal muscle; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-MYPN Polyclonal Antibody, Unconjugated(bs-10366R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-10366R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10366R |
| 英文名称 | MYPN Rabbit pAb |
| 中文名称 | 肌钯蛋白抗体 |
| 英文别名 | 145 kDa sarcomeric protein; MYOP; Myopalladin; MYPN; MYPN_HUMAN; sarcomeric protein myopalladin. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MYPN |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 145 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Motor Proteins > Myosin |
| 亚基 | Interacts with TTN/titin, NEB, NEBL, ACTN2 and CARP. |
| 亚细胞定位 | Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere. Note=Bound to sarcomere both at the Z-line periphery and in the central I-band region. |
| 组织特异性 | Expressed in adult skeletal muscle and fetal heart. |
| 相似性 | Belongs to the myotilin/palladin family. Contains 5 Ig-like (immunoglobulin-like) domains. |
| 功能 | Component of the sarcomere that tethers together nebulin (skeletal muscle) and nebulette (cardiac muscle) to alpha-actinin, at the Z lines. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-MYPN Polyclonal Antibody, Unconjugated(bs-10366R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-MYPN Polyclonal Antibody, Unconjugated(bs-10366R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
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MYPN | MYOP Rabbit pAb(bs-10366R)-50ul/100ul/200ul
￥1180 - 2800
