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- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1201R |
| 英文名称 | PAX8 Rabbit pAb |
| 中文名称 | 配对盒基因8抗体 |
| 英文别名 | Paired Box Gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired Domain Gene 8; PAX 8; PAX-8; PAX8_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Cow, Sheep, Goat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PAX8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families > PAX Neuroscience > Sensory System > Auditory system |
| 亚基 | Interacts with WWTR1. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in the excretory system, thyroid gland and Wilms tumors. |
| 翻译后修饰 | S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. |
| 相似性 | Contains 1 paired domain. |
| 功能 | Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Protocol
1.Jurkat cells were washed twice with phosphate-buffered saline (PBS). 2. An equivalent amount of pre-warmed 4% Paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAX8 Polyclonal Antibody, Unconjugated(bs-1201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Concentration:1:100
Host/Isotype:Rabbit/IgG(blue line)
Flow cytometric analysis of Rabbit IgG isotype control on HT-29(blue) compared with primary antibody(Cat#: bs-1021R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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文献和实验[IF={{ 4.872 }}] {Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats 2.5Ecotoxicol Environ Saf.2021 Jan 15;208:111720.} {IHC,WB} {Rat}
[IF={{ 4.711 }}] {Xiuzhen Du. et al. Membranous and nuclear staining of CLDN18 in HPV-independent and HPV-associated endocervical adenocarcinomas. CANCER MED-US. 2022 Jul;:} {IHC} {Human}
[IF={{ 4.223 }}] {Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13} {WB,IHC} {Rat}
[IF={{ 2.34 }}] {Huang et al. Upregulation of TSHR, TTF-1, and PAX8 in Nodular Goiter Is Associated with Iodine Deficiency in the Follicular Lumen. (2016) Int.J.Endocrinol. 2016:2492450} {IHC,WB} {Human}
[IF={{ 1.38 }}] {Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014).} {Other} {="Human"}
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