Tissue/cell: mouse spleen; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRF4 Polyclonal Antibody, Unconjugated(bs-2500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-2500R
- 2025年10月16日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2500R |
| 英文名称 | IRF4 Rabbit pAb |
| 中文名称 | 干扰素调节因子4抗体 |
| 英文别名 | Interferon regulatory factor 4; IRF 4; IRF4; IRF-4; LSIRF; Lymphocyte specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM 1; NF EM5; NFEM5; OTTHUMP00000017905; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP; IRF4_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Chicken, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IRF-4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Transcription factors Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Proto-oncogenes Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚基 | Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with SPIB and DEF6. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Lymphoid cells. |
| 翻译后修饰 | Phosphorylation by ROCK2 regulates IL-17 and IL-21 production (By similarity). |
| 相似性 | Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain. |
| 功能 | Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 2.8 }}] {Zhuang Cuicui. et al. Lycopene promoted M2 macrophage polarization via inhibition of NOTCH1-PI3K-mTOR-NF-κB-JMJD3-IRF4 pathway in response to Escherichia coli infection in J744A.1 cells. ARCH MICROBIOL. 2024 Jun;206(6):1-11} {WB} {Mouse}
[IF={{ 2.264 }}] {Zhiyu Zhang. et al. CCL17 blockade by CCL17mAb/GSK-J4 ameliorates hyperalgesia in a rat model of postoperative pain. BIOL PHARM BULL. 2023 Feb 15} {IF} {Rat}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
分子 -1 (rabbit ICAM-1) ELISA 48T/96T 2000/3000 进口分装 F20170 兔干扰素 -r(rabbit IFN-r) ELISA 48T/96T 1500/2500 进口分装 F20180 兔白介素
分装 F0110 干扰素-r 受体 (IFN-rR) ELISA 48T/96T 1200/2000 进口分装 F0111 抗干扰素- a 抗体 (anti-IFN- a b) ELISA 48T/96T 1200/2000 进口分装 F0112 胰岛素样生长因子-1 (IGF-1) ELISA 48T/96T 1500/2500 进口分装 F0113 胰岛素样生长因子-2 (IGF-2) ELISA 48T/96T 1500/2500
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IRF4 Rabbit pAb(bs-2500R)-50ul/100ul/200ul
￥1180 - 2800
