ADAM8 | CD156a Rabbit pAb(bs-4

Blank control: Mouse splenocytes(blue)
_x000D_ Isotype Control Antibody: Rabbit IgG(orange) ; _x000D_ Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), _x000D_ Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; _x000D_ Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green)._x000D_

Blank control: A431.
_x000D_ Primary Antibody (green line): Rabbit Anti-ADAM8 antibody (bs-4195R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_

Black line : Positive blank control A431); Negative blank control (HepG2）
_x000D_ Green line : Primary Antibody (Rabbit Anti-ADAM8 antibody (bs-4195R) )
_x000D_ Orange line：Isotype Control Antibody (Rabbit IgG) .
_x000D_ Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
_x000D_ A431（Positive）and HepG2（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody(bs-4195R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange)._x000D_

4% P‌‌araformaldehyde-fixed Raji (H) cell; Antibody incubation with (ADAM8) polyclonal Antibody, unconjugated (bs-4195R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.