Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated(bs-1146R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-1146R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1146R |
| 英文名称 | NRAS Rabbit pAb |
| 中文名称 | 原癌基因N-Ras抗体 |
| 英文别名 | GTPase NRas; OTTMUSP00000023521; ALPS4; AV095280; HRAS1; N ras; N ras protein part 4; Neuroblastoma RAS viral(v ras) oncogene homolog; NRAS1; OTTHUMP00000013879; Transforming protein N Ras; v ras neuroblastoma RAS viral oncogene homolog. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, human cervical carcinoma (Mouse, Rat, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human N-Ras |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 21 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Signal transducers Cancer > Signal transduction > G protein signaling > Small G proteins > Ras family Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Ras Family |
| 亚基 | Interacts (active GTP-bound form preferentially) with RGS14. Interacts (active GTP-bound form) with RASSF7. |
| 亚细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus. |
| 组织特异性 | Widely expressed. |
| 翻译后修饰 | Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs). |
| 相似性 | Belongs to the small GTPase superfamily. Ras family. |
| 功能 | Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | N-RAS (neuroblastoma RAS viral oncogene homolog) analogously to other GTP-binding proteins (such as Translation Elongation Factor EFTu or signal transducing G-Proteins) RAS proteins are involved in signal transduction pathways, ras gene family is part of the ras superfamily including the mammalian RAS, RAL, RAC, RHO, RAP, and RAB gene families and the yeast homologs like SEC4 and YPT1 genes; genes encode small monomeric proteins of low molecular mass (20-30 kDa) which share at least 30% homology to RAS proteins. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human gastric carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated(bs-1146R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated(bs-1146R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C.
_x000D_
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Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
Ballard, J.L., Peeva, V.K., deSilva, C.J., Lynch, J.L., and Swanson, N.R., 2007. Comparison of Alexa Fluor and CyDye for practical DNA microarray use. Mol. Biotechnol. 36:175‐183.
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NRAS | N-Ras Rabbit pAb(bs-1146R)-50ul/100ul/200ul
￥1180 - 2800
