Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-REC8 antibody (bs-7723R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-7723R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7723R |
| 英文名称 | REC8 Rabbit pAb |
| 中文名称 | 减数分裂重组蛋白家族REC8抗体 |
| 英文别名 | Cohesin Rec8p; Meiotic recombination protein REC8 homolog; Meiotic recombination protein REC8-like 1; rec8; REC8 homolog(yeast); REC8_HUMAN; REC8L; Rec8L1; Rec8p. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Horse, Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human REC8 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 60 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Cell Cycle > Cell Division > Chromatid Cohesion Epigenetics and Nuclear Signaling > Cell cycle > Chromosome Structure > Chromatid Cohesion |
| 亚基 | Interacts (phosphorylated and unphosphorylated form) with SMC3. Interacts with SYCP3. Interacts (phosphorylated and unphosphorylated form) with SMC1B. Does not interact with SMC1A. Interacts with RAD51. |
| 亚细胞定位 | Nucleus. Chromosome. |
| 组织特异性 | Expressed in testis and thymus. |
| 相似性 | Belongs to the rad21 family. |
| 功能 | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Sample: HT29 Cell (Human) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD
Primary: Anti-REC8 (bs-7723R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 65 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
Thymus (Mouse) Lysate at 40 ug
Primary: Anti-REC8 (bs-7723R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
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文献和实验该产品被引用文献
[IF={{ 6.831 }}] {Shangren Wang. et al. Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction. 2022 Mar 17} {WB} {Rat}
[IF={{ 2.7 }}] {Mitsuru Nago. et al.Sod1deficiency in mouse oocytes during in vitro maturation increases chromosome segregation errors with a reduced BUBR1 at kinetochore.REPRODUCTIVE MEDICINE AND BIOLOGY.2025 Jan 22;24(1):e12622.} {Western blot} {Mouse}
[IF={{ 1.635 }}] {Shimoi G et al.Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes.(2018)J Reprod Dev.} {WB} {Mouse}
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REC8 Rabbit pAb(bs-7723R)-50ul/100ul/200ul
￥1180 - 2800
