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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4630R |
| 英文名称 | phospho-ROCK1 (Thr455+Ser456) Rabbit pAb |
| 中文名称 | 磷酸化Rho相关蛋白激酶1抗体 |
| 英文别名 | p-ROCK1(Thr455/Ser456); p160 ROCK1; p160ROCK; Renal carcinoma antigen NY REN 35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho-associated coiled-coil containing protein kinase 1; ROCK1_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455+Ser456 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 158 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell cycle > Cell division Cell Biology > Cell Cycle > Cell Division > Centromere Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Assembly Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. |
| 亚细胞定位 | Cytoplasm. Cytoplasm, cytoskeleton, centrosome, centriole (By similarity). Golgi apparatus membrane; Peripheral membrane protein. Cell projection, bleb. Note=Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes. |
| 组织特异性 | Detected in blood platelets. |
| 翻译后修饰 | Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR._x000D_
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. _x000D_ Contains 1 AGC-kinase C-terminal domain. _x000D_ Contains 1 PH domain. _x000D_ Contains 1 phorbol-ester/DAG-type zinc finger. _x000D_ Contains 1 protein kinase domain. _x000D_ Contains 1 REM (Hr1) repeat. |
| 功能 | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-phospho-ROCK1(Thr455+Ser456) (bs-4630R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 158 kD
_x000D_ Observed band size: 158 kD
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-phospho-ROCK1(Thr455/Ser456) Polyclonal Antibody, Unconjugated(bs-4630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Primary Antibody:Rabbit Anti- phospho-ROCK1(Thr455+Ser456) antibody(bs-4630R), Dilution:1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ Primary Antibody (green line): Rabbit Anti-phospho-ROCK1 (Thr455+Ser456) antibody (bs-4630R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）：Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
_x000D_ Primary Antibody (green line): Rabbit Anti-phospho-ROCK1 (Thr455+Ser456) antibody (bs-4630R)
_x000D_ Dilution: 1ug/Test;
_x000D_ Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）：Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验[IF=6.7] Zhizhongbin Wu. et al. Long term Coptidis Rhizoma intake induce gastrointestinal emptying inhibition and colon barrier weaken via bitter taste receptors activation in mice. PHYTOMEDICINE. 2024 Nov;:156292 WB Human
[IF=6.162] Zhou Y et al. ROCK2 Confers Acquired Gemcitabine Resistance in Pancreatic Cancer Cells by Upregulating Transcription Factor ZEB1. Cancers (Basel). 2019 Nov 27;11(12). pii: E1881. WB Human
[IF=42.5] Yan Hu. et al. Selective targeting of endothelial and perivascular angiocrine ROCK2 treats liver fibrosis. CELL. 2026 Mar mIF Human
[IF=4.566] Xu Jiahui. et al. Temperature and Growth Selection Effects on Proliferation, Differentiation, and Adipogenic Potential of Turkey Myogenic Satellite Cells Through Frizzled-7-Mediated Wnt Planar Cell Polarity Pathway. FRONT PHYSIOL. 2022 May;0:889 WB Chicken
[IF=4.15] Nan Wang. et al. Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway. INT J BIOCHEM CELL B. 2013 Jul;45:1447 WB ="Rat"
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
phosphorylation in rabbit reticulocyte lysates. Proc. Natl. Acad. Sci. U.S.A. 79:7092‐7096. Fare, T.L., Coffey, E.M., Dai, H., He, Y.D., Kessler, D.A., Kilian, K.A., Koch, J.E
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. Berton, T.R., Matsumoto, T., Page, A., Conti, C.J., Deng, C.X., Jorcano, J.L., and Johnson, D.G. 2003. Tumor formation in mice with conditional inactivation of Brca1 in epithelial tissues. Oncogene 22:5415
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
. Deng, G.G., Martin‐McNulty, B., Sukovich, D.A., Freay, A., Halks‐Miller, M., Thinnes, T., Loskutoff, D.J., Carmeliet, P., Dole, W.P., and Wang, Y.X. 2003. Urokinase‐type plasminogen activator plays a critical
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