Sample: A549 Cell (Human) Lysate at 40 ug
Primary: Anti- LAMP3 (bs-2556R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
- ￥1180 - 2800
- Bioss
- bs-2556R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2556R |
| 英文名称 | LAMP3 Rabbit pAb |
| 中文名称 | 溶酶体相关膜蛋白3抗体 |
| 英文别名 | DC-lysosome-associated membrane glycoprotein; CD208; CD208 antigen; DC LAMP; DCLAMP; DC-LAMP; LAMP; LAMP 3; LAMP-3; LAMP3; Lysosomal associated membrane protein 3; Protein TSC403; TSC403; DC lysosome associated membrane glycoprotein; LAMP3_HUMAN; Lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LAMP-3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Stem Cells > Hematopoietic Progenitors > Myeloid > Dendritic Cell Lineage Tags & Cell Markers > Subcellular Markers > Organelles > Lysosome |
| 亚细胞定位 | Lysosome membrane. |
| 组织特异性 | Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues. |
| 相似性 | Belongs to the LAMP family. |
| 功能 | Might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | LAMP3 might change the lysosome function after the transfer of peptide MHC class II molecules to the surface of dendritic cells. It's over expression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer. Expressed in lymphoid organs and dendritic cells and lung. Up regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1ug/Test} |
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-LAMP3 antibody (bs-2556R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-LAMP3 antibody (bs-2556R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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LAMP3 Rabbit pAb(bs-2556R)-50ul/100ul/200ul
￥1180 - 2800
