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PRCP Rabbit pAb(bs-1873R)-50ul

/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-1873R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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    • 规格

      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-1873R
    英文名称PRCP Rabbit pAb
    中文名称脯氨酰羧肽酶抗体
    英文别名prolylcarboxypeptidase; Angiotensinase C; HUMPCP; Lysosomal carboxypeptidase C; Lysosomal Pro x carboxypeptidase; PCP; Proline carboxypeptidase.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse (Human, Rat, Dog, Cow, Horse)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PRCP
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量55 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Blood > Blood Pressure regulation

    Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases

    亚基Homodimer.
    亚细胞定位Lysosome.
    组织特异性Highest levels in placenta, lung and liver. Also present in heart, brain, pancreas and kidney.
    相似性Belongs to the peptidase S28 family.
    功能Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料PRCP belongs to the peptidase S28 family. It cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRCP) Polyclonal Antibody, Unconjugated (bs-1873R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
    产品细节图片2
    Sample: bone (Mouse) Lysate at 40 ug
    Primary: Anti-PRCP(bs-1873R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55 kD
    Observed band size: 55 kD

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    ¥1180 - 2800