Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GABA Polyclonal Antibody, Unconjugated(bs-2252R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-2252R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2252R |
| 英文名称 | GABA Rabbit pAb |
| 中文名称 | γ氨基丁酸抗体 |
| 英文别名 | gamma-aminobutyric acid; gamma aminobutyric acid; 4 aminobutanoic acid; gamma amino butyric acid; γ－aminobutyric acid(GABA). |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated GABA |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 0.1031 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABA acts at inhibitory synapses in the brain and spinal cord. Inhibition is provoked by GABA binding resulting in hyperpolarization of the synaptic transmembrane potential of the affected neuron. GABA binding causes ion channels to open allowing either the flow of chloride or potassium ions into or out of the cell. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABA) Polyclonal Antibody, Unconjugated (bs-2252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 9.3 }}] {Wang Lu-Ying. et al. NLRP3–GABA signaling pathway contributes to the pathogenesis of impulsive-like behaviors and cognitive deficits in aged mice. J NEUROINFLAMM. 2023 Dec;20(1):1-15} {IF} {Mouse}
[IF={{ 7.9 }}] {Dong-Xue Zhang. et al. Icariin mitigates anxiety-like behaviors induced by hemorrhagic shock and resuscitation via inhibiting of astrocytic activation. PHYTOMEDICINE. 2024 Jun;128:155507} {IF} {Mouse}
[IF={{ 5.4 }}] {Taotao Li. et al. Rosemary (Rosmarinus officinalis L.) hydrosol based on serotonergic synapse for insomnia. J ETHNOPHARMACOL. 2023 Jul;:116984} {IHC,WB} {Rat}
[IF={{ 5.25 }}] {Li-Min Zhang. et al. Subanesthetic dose of S-ketamine improved cognitive dysfunction via the inhibition of hippocampal astrocytosis in a mouse model of post-stroke chronic stress. J PSYCHIATR RES. 2023 Feb;158:1} {IF} {Mouse}
[IF={{ 3.8 }}] {Wang Chunjing. et al. Genetically engineered BMSCs promote dopamine secretion and ameliorate motor dysfunction in a Parkinson’s disease rat model. SCI REP-UK. 2025 Apr;15(1):1-15} {IHC,FC} {Rat}
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Majewska, M.D., Harrison, N.L., Schwartz, R.D., Barker, J.L., and Paul, S.M. 1986. Steroid hormone metabolites are barbiturate‐like modulators of the GABA receptor. Science 232:1004‐1007
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GABA Rabbit pAb(bs-2252R)-50ul/100ul/200ul
￥1180 - 2800
