Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-Cygb Polyclonal Antibody, Unconjugated(bs-0590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- ￥1180 - 2800
- Bioss
- bs-0590R
- 2026年10月13日
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- 文献和实验
- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0590R |
| 英文名称 | CYGB Rabbit pAb |
| 中文名称 | 细胞球蛋白抗体 |
| 英文别名 | Cytoglobin; HGB; STAP; Histoglobin; HGb; cell activation-associated protein; CYGB_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Chicken, Dog, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human cytoglobin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 21 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood > Serum Proteins |
| 亚基 | Homodimer; disulfide-linked. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | Ubiquitously expressed. Highest expression in heart, stomach, bladder and small intestine. |
| 相似性 | Belongs to the globin family. |
| 功能 | May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cygb is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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CYGB | Cytoglobin Rabbit pAb(bs-0590R)-50ul/100ul/200ul
￥1180 - 2800
