Blank control:U937.
_x000D_
Primary Antibody (green line): Rabbit Anti-Caspase 4 p20 antibody (bs-0082R)
_x000D_
Dilution: 2μg /10^6 cells;
_x000D_
Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_
Secondary Antibody : Goat anti-rabbit IgG-PE
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Dilution: 1μg /test.
_x000D_
Protocol
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The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-0082R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0082R |
| 英文名称 | CASP4 Rabbit pAb |
| 中文名称 | 半胱胺酸蛋白酶蛋白-4抗体 |
| 英文别名 | Caspase-4 subunit p20; Caspase-11; CASP-11; Protease ICH-3; Apoptosis related cysteine peptidase; Apoptotic cysteine protease Mih1/TX; CASP 4; CASP-4; CASP4; CASP4_HUMAN; CASP4_MOUSE; Caspase 4 apoptosis related cysteine peptidase; Caspase-4 subunit 2; Caspase4; ICE(rel) II; ICE(rel)-II; ICE(rel)II; ICEREL II; ICERELII; ICH 2; ICH 2 protease; ICH2; Mih1/TX; Mih1/TX; Protease ICH-2; Protease TX; TX; TX protease; EC:3.4.22.64. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Rabbit, Zebrafish) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse Caspase-4 subunit p20 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 20/43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Apoptosis > Intracellular > Caspases etc > Caspases |
| 亚基 | Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. |
| 组织特异性 | Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. |
| 翻译后修饰 | The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. |
| 相似性 | Belongs to the peptidase C14A family. Contains 1 CARD domain. |
| 功能 | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
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Casp4 | Caspase-11 Rabbit pAb(bs-0082R)-50ul/100ul/200ul
￥1180 - 2800
