Sample: Mcf-7 Cell Lysate at 40 ug
_x000D_
Primary: Anti-Muscarinic Acetylcholine receptor (bs-1289R) at 1/300 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 65 kD
_x000D_
Observed band size: 65 kD
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- ￥1180 - 2800
- Bioss
- bs-1289R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1289R |
| 英文名称 | Muscarinic Acetylcholine receptor M3 Rabbit pAb |
| 中文名称 | 毒蕈碱型乙酰胆碱受体M3抗体 |
| 英文别名 | Muscarinic Acetylcholine receptor M3; cholinergic receptor, muscarinic 3; CHRM 3; CHRM3; HM 3; HM3; HM3; EGBRS; M3 muscarinic receptor; muscarinic 3; m3 muscarinic acetylcholine receptor; ACM3_HUMAN; muscarinic receptor M3; muscarinic cholinergic m3 receptor; muscarinic m3 receptor; Acetylcholine receptor(M3). |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, human lung carcinoma (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ChRM3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 65 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Acetylcholine Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Muscarinic Receptors |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. |
| 功能 | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-ChRM3/Acetylcholine receptor(M3) Polyclonal Antibody, Unconjugated (bs-1289R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-ChRM3/Acetylcholine receptor(M3) Polyclonal Antibody, Unconjugated (bs-1289R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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CHRM3 Rabbit pAb(bs-1289R)-50ul/100ul/200ul
￥1180 - 2800
