Sample: Mcf-7 Cell Lysate at 40 ug
Primary: Anti-Muscarinic Acetylcholine receptor (bs-1289R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
- ￥1180 - 2800
- Bioss
- bs-1289R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1289R |
| 英文名称 | Muscarinic Acetylcholine receptor M3 Rabbit pAb |
| 中文名称 | 毒蕈碱型乙酰胆碱受体M3抗体 |
| 英文别名 | Muscarinic Acetylcholine receptor M3; cholinergic receptor, muscarinic 3; CHRM 3; CHRM3; HM 3; HM3; HM3; EGBRS; M3 muscarinic receptor; muscarinic 3; m3 muscarinic acetylcholine receptor; ACM3_HUMAN; muscarinic receptor M3; muscarinic cholinergic m3 receptor; muscarinic m3 receptor; Acetylcholine receptor(M3). |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, human lung carcinoma (Mouse, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ChRM3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 65 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Acetylcholine Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Muscarinic Receptors |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily. |
| 功能 | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ChRM3/Acetylcholine receptor(M3) Polyclonal Antibody, Unconjugated (bs-1289R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ChRM3/Acetylcholine receptor(M3) Polyclonal Antibody, Unconjugated (bs-1289R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验该产品被引用文献
[IF={{ 5.753 }}] {Xue Wang. et al. The intact parasympathetic nerve promotes submandibular gland regeneration through ductal cell proliferation. 2021 Jun 07} {WB} {Rat}
[IF={{ 3.73 }}] {Chen, Bing, et al. "An ENU-Induced Mutation of Nrg1 Causes Dilated Pupils and a Reduction in Muscarinic Receptors in the Sphincter Pupillae." PloS one 6.9 (2011): e25176.} {IHC} {="Mouse"}
[IF={{ 3.448 }}] {Huang N et al. Chrm3 protects against acinar cell necrosis by stabilizing caspase‐8 expression in severe acute pancreatitis mice model. J Cell Biochem. 2019 Nov 6.} {WB,IF} {Mouse}
[IF={{ 2.92 }}] {Luan Jing. et al. Inhibition of CHRM3 Alleviates Necrosis Via the MAPK-p38/miR-31-5p/RIP3 Axis in l-Arginine–Induced Severe Acute Pancreatitis. Pancreas. 2020 Nov;49(10):1335-1341} {WB} {Mouse}
[IF={{ 2.624 }}] {Liyang Tong. et al. Topical bendazol inhibits experimental myopia progression and decreases the ocular accumulation of HIF‐1α protein in young rabbits. Ophthal Physl Opt. 2020 Sep;40(5):567-576} {WB} {Rat}
相关实验
Overview of Receptor Allosterism
Figure 1.21.1 Allosterism between a receptor and its G protein: the effects of GTP on the competition between [3 H] N ‐methylscopolamine and the agonist, carbachol, at the muscarinic acetylcholine M2 receptor in rat myocardial membranes. Competition
Dowling, M.R. and Charlton, S.J. 2006. Quantifying the association and dissociation rates of unlabelled antagonists at the muscarinic M3 receptor. Br. J. Pharmacol. 148:927‐937.
. and El‐Fakahany, E.E. 1991. Allosteric antagonists of the muscarinic acetylcholine receptor. Biochem. Pharmacol. 42:199‐205. Leppik, R.A., Lazareno, S., Mynett
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Muscarinic Acetylcholine receptor M3 Rabbit pAb(bs-1289R)-50ul/100ul/200ul
￥1180 - 2800
