Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-1002R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Histone H3 (mono methyl K9) Mouse mAb, Biotin conjugated(bsm-33116M-Bio)-100ul
￥2980FKRP Rabbit pAb, HRP conjugated(bs-16097R-HRP)-100ul
￥2980
Placental Lactogen Recombinant Rabbit mAb(bsm-60796R)-50ul/100ul/25ul
￥800E2F1 Rabbit pAb, PE-Cy5.5 conjugated(bs-23185R-PE-Cy5.5)-100ul
￥2980
Monoamine oxidase A+B Rabbit pAb(bs-11890R)-50ul/100ul/200ul
￥1180
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1002R |
| 英文名称 | MGMT Rabbit pAb |
| 中文名称 | O6甲基鸟嘌呤DNA甲基转移酶抗体 |
| 英文别名 | 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT: O-6 methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6 methylguanine DNA alkyltransferase; O-6-methylguanine-DNA methyltransferase; O6 methylguanine DNA methyltransferase; MGMT_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MGMT |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > Direct Chemical Reversal Epigenetics and Nuclear Signaling > DNA methylation > DNMTs |
| 亚细胞定位 | Nucleus. |
| 相似性 | Belongs to the MGMT family. |
| 功能 | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MGMT Polyclonal Antibody, Unconjugated(bs-1002R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 3.61 }}] {Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014).} {WB} {="Human"}
[IF={{ 2.98 }}] {Li et al. Effects of low-intensity ultrasound combined with low-dose carboplatin in an orthotopic hamster model of tongue cancer: A preclinical study. (2018) Oncol.Rep. 39:1609-1618} {IHC,WB} {Hamsters}
[IF={{ 2.886 }}] {Yusi Liu. et al. Synergistic Effects of Resveratrol and Temozolomide Against Glioblastoma Cells: Underlying Mechanism and Therapeutic Implications. Cancer Manag Res. 2020; 12: 8341–8354} {ICC} {Rat, Human}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
of tumor cells flowing in a parallel plate chamber to determine their adhesion stabilization lag time. Cell. Motil. Cytoskeleton. 26, 88-98, doi:10.1002/cm.970260109 (1993). 21. Wiese, G., Barthel, S.R., & Dimitroff, C.J. Analysis of Physiologic E
Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
) Ki67 (rabbit monoclonal, Abcam, cat. no. 16667, 1 mg/ml, 1:300) α‐Sarcomeric actinin (α‐SA, mouse monoclonal, Sigma, cat. no. A7811, 1 mg/ml, 1:250
技术资料暂无技术资料 索取技术资料
文献支持
MGMT Rabbit pAb(bs-1002R)-50ul/100ul/200ul
￥1180 - 2800
