Tissue/cell: rat skin tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PER2/Period circadian protein 2 Polyclonal Antibody, Unconjugated(bs-3927R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-3927R
- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3927R |
| 英文名称 | PER2 Rabbit pAb |
| 中文名称 | 节律蛋白2抗体 |
| 英文别名 | Circadian clock protein PERIOD 2; FASPS; hPER 2; hPER2; KIAA0347; PER 2; Period 2; Period 2 isoform 1; Period circadian protein 2; Period circadian protein homolog 2; Period homolog 2; Period2; FASPS; PER2_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PER2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 138 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood > Blood Pressure regulation Cardiovascular > Vasculature > Endothelium Epigenetics and Nuclear Signaling > Transcription > Domain Families > HLH / Leucine Zipper > HLH Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Neuroscience > Neurology process > Circadian Rhythm Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Homodimer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER3, and through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts, via its second PAS domain, with TIMELESS in vitro. Interacts with NFIL3 (By similarity). Interacts with PML (By similarity). |
| 亚细胞定位 | Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. |
| 组织特异性 | Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung. |
| 翻译后修饰 | Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation. |
| 相似性 | Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains. |
| 功能 | Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK NPAS2-BMAL1 |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | PER2, a mammalian homologue of the Drosophila period gene, shares a 40% homology with PER1 including the protein dimerization PAS domain. PER2 is a circadian regulator that may act as a transcription factor. It behaves as a negative element in circadian transcriptional loop. PER2 does not appear to bind DNA, suggesting indirect transcriptional inhibition. Expression oscillates with a 24 hour rhythm in the suprachiasmatic nucleus (SCN) and the whole eyes. Oscillations are maintained under constant darkness and are responsive to changes of the light/dark cycles. There is a 4 hour time delay between PER1 and PER2 oscillations. The expression rhythms appear to originate from retina. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER2) Polyclonal Antibody, Unconjugated (bs-3927R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PER2) Polyclonal Antibody, Unconjugated (bs-3927R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF={{ 6.354 }}] {Mishra, Rakesh. et al. Reversible dysregulation of renal circadian rhythm in lupus nephritis. Mol Med. 2021 Dec;27(1):1-12} {ELISA} {mouse}
[IF={{ 5.227 }}] {Yi-Ting Chen. et al. Early detection of the initial stages of LED light-triggered non-alcoholic fatty liver disease by wax physisorption kinetics-Fourier transform infrared imaging. ANALYST. 2022 Dec;:} {IHC} {Mouse}
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PER2 Rabbit pAb(bs-3927R)-50ul/100ul/200ul
￥1180 - 2800
