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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-33396M |
| 英文名称 | ATG7/APG7 Mouse mAb |
| 中文名称 | 自噬相关蛋白7单克隆抗体 |
| 英文别名 | Apg 7; ATG7; APG7 autophagy 7 like; APG7 autophagy 7-like(S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7L; ATG 7; atg7; ATG7 autophagy related 7 homolog(S. cerevisiae); ATG7 autophagy related 7 homolog; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7(yeast); DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; ATG7_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat) |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human ATG7 Protein |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 78 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cell Death > Autophagy > APG gene products Cancer > Cell Death > Autophagy > Signal Transduction Cancer > Signal transduction > Autophagy Cardiovascular > Heart > Autophagy > APG gene products Cell Biology > Apoptosis > Intracellular > Associated Proteins Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ub-like Proteins Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy Metabolism > Pathways and Processes > Metabolism processes > Autophagy and mitophagy > APG gene products Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitophagy fission and fusion Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. |
| 亚细胞定位 | Cytoplasm (Probable). |
| 组织特异性 | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. |
| 相似性 | Belongs to the ATG7 family. |
| 功能 | Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
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Hybrid Resistance by Mouse NK Cells In Vitro
A purified NK cells to kill H2d target cells can be reversed by adding MAb reagents that bind to either Ly49A or H2-Dd . We and others have extended these early findings to the Ly49C and I subsets of NK cells, and have recently detected positive signaling
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