HPV16 E6 + HPV18 E6 Rabbit pAb

Tissue/cell: human colon carcinoma; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody, Unconjugated(bs-1719R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Sample:
Lane 1: Recombinant HPV16 E6 protein, DsbC & His(bs-49102P) 0.1ug
Lane 2: Recombinant HPV16 E6 protein, DsbC & His(bs-49102P) 0.05ug
Primary: Anti-HPV16 E6 + HPV18 E6 (bs-1719R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 50 kDa

Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-HPV16 E6 + HPV18 E6 antibody (bs-1719R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.