Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-ROCK2 (bs-1205R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kD
Observed band size: 161 kD
- ￥1180 - 2800
- Bioss
- bs-1205R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1205R |
| 英文名称 | ROCK2 Rabbit pAb |
| 中文名称 | Rho相关蛋白激酶2抗体 |
| 英文别名 | p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; coiled-coil-containing protein kinase 2; KIAA0619; Rho associated, coiled coil containing protein kinase II; Rho-associated; Rho-associated protein kinase 2; Rock2; ROCK2_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ROCK2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 161 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction > Protein phosphorylation > Serine/threonine kinases Cell Biology > Apoptosis > Intracellular > Kinases Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚基 | Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1. |
| 亚细胞定位 | Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Tyr-722 reduces its binding to RHOA and iscrucial for focal adhesion dynamics. Dephosphorylation by PTPN11stimulates its RHOA binding activity. |
| 相似性 | Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family.
Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. |
| 功能 | Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ROCK2 Polyclonal Antibody, Unconjugated(bs-1205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ROCK2 Polyclonal Antibody, Unconjugated(bs-1205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lane 1: Mouse Heart Lysates
Lane 2: Mouse Skeletal muscle Lysates
Primary: Anti-ROCK2 (bs-1205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161kDa
Observed band size: 180kDa
Lane 1: Mouse Heart Lysates
Lane 2: Mouse Skeletal muscle Lysates
Primary: Anti-ROCK2 (bs-1205R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161kDa
Observed band size: 180kDa
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 4.26 }}] {Zhu et al. Design and evaluation of EphrinA1 mutants with cerebral protective effect. (2017) Sci.Re. 7:1881} {WB} {Human,Mouse}
[IF={{ 4.2 }}] {Shangtao Wang. et al. The Combination of Gastrodin and Gallic Acid Synergistically Attenuates AngII-Induced Apoptosis and Inflammation via Regulation of Sphingolipid Metabolism. J INFLAMM RES. 2024 Oct 03} {WB} {Human}
[IF={{ 4.19 }}] {He, Hongpeng, et al. "Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells." Cellular Signalling (2017).} {WB} {="Human"}
[IF={{ 4.15 }}] {Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).} {WB} {="Rat"}
[IF={{ 4.12 }}] {Rothschild et al. ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy. (2017) Sci.Rep. 7:8834} {IF} {Rat}
技术资料暂无技术资料 索取技术资料
文献支持
ROCK2 Rabbit pAb(bs-1205R)-50ul/100ul/200ul
￥1180 - 2800
