- ￥1180 - 2800
- Bioss
- bs-3759R
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
MT2A Rabbit pAb, BF594 conjugated(bs-1328R-BF594)-100ul
￥2980Beta galactosidase Rabbit pAb, BF680 conjugated(bs-4631R-BF680)-100ul
￥2980ERBB2/HER2 Rabbit pAb, Cy7 conjugated(bs-0125R-Cy7)-100ul
￥2980
Histone H3 (Tri Methyl K36) Recombinant Rabbit mAb(bsm-60113R)-50ul/100ul/25ul
￥800phospho-Hsp90 beta (Ser226) Rabbit pAb, Cy5 conjugated(bs-18074R-Cy5)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3759R |
| 英文名称 | S100A4 Rabbit pAb |
| 中文名称 | FSP-1抗体 |
| 英文别名 | FSP1; FSP-1; FSP 1; 18A2; 42A; calcium Placental protein; Calcium protein; Calvasculin; CAPL; Fibroblast specific protein 1; FSP1; Leukemia multidrug resistance associated protein; Malignant transformation suppression 1; Metastasin; MTS1; Mts1 protein; Murine placental homolog; OTTHUMP00000015467; OTTHUMP00000015468; P9KA; PEL98; Placental calcium binding protein; Placental calcium-binding protein; Protein Mts1; Protein S100 A4; Protein S100-A4; S100 calcium binding protein A4(calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4; S100 calcium-binding protein A4; S100a4; S10A4_HUMAN |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse, Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human S100A4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 12 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > ECM > Extracellular matrix Cardiovascular > Angiogenesis > Angiogenic Factors Cardiovascular > Heart > Cardiogenesis > Myocardial regeneration Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Binding Proteins |
| 亚基 | Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode. |
| 组织特异性 | Ubiquitously expressed. |
| 相似性 | Belongs to the S-100 family. Contains 2 EF-hand domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-S100A4 Polyclonal Antibody, Unconjugated(bs-3759R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Primary Antibody (green line): Rabbit Anti-S100A4 antibody (bs-3759R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验[IF={{ 7.419 }}] {Honghua Yue. et al. Colchicine: Emerging therapeutic effects on atrial fibrillation by alleviating myocardial fibrosis in a rat model. BIOMED PHARMACOTHER. 2022 Oct;154:113573} {IF} {Rat}
[IF={{ 6.543 }}] {Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022} {WB,IF} {Rat,Human}
[IF={{ 5.2 }}] {Ruyan Lv. et al. Fibroblast to macrophage-like cell transition in renal inflammatory injury through the MR/CSF1 pathway induced by aldosterone. LIFE SCI. 2025 Apr;:123627} {FC} {Rat}
[IF={{ 4.8 }}] {Ruilin Wang. et al. Decreased expression of hyaluronan synthase and loss of hyaluronan-rich cells in the anterior tibial fascia of the rat model of chemotherapy-induced peripheral neuropathy. Pain Reports. 2023 Jul-Aug; 8(4): e1088} {IF} {Rat}
[IF={{ 3.947 }}] {Li Tao. et al. Epigallocatechin-3-Gallate Inhibits Atrial Fibrosis and Reduces the Occurrence and Maintenance of Atrial Fibrillation and its Possible Mechanisms. CARDIOVASC DRUG THER. 2023 Mar;:1-22} {ICC} {Rat}
技术资料暂无技术资料 索取技术资料
