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- bs-0311R
- 2025年10月10日
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- 规格：
100ul/200ul
| 规格： | 100ul | 产品价格： | ￥1580.0 |
|---|---|---|---|
| 规格： | 200ul | 产品价格： | ￥2480.0 |
| 产品编号 | bs-0311R |
| 英文名称 | Rabbit Anti-Biotin |
| 中文名称 | 兔抗生物素抗体 |
| 英文别名 | Vitamin B4. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:2000-10000 Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Biotin |
| 亚型 | IgG |
| 性状 | Lyophilized or Liqui |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:2000-10000} |
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文献和实验[IF={{ 8.008 }}] {Yepei Huang. et al. Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling. ANAL CHEM. 2022;XXXX(XXX):XXX-XXX} {WB} {Human}
[IF={{ 7.7 }}] {Xuecong Ren. et al. MIRO-1 interacts with VDAC-1 to regulate mitochondrial membrane potential in Caenorhabditis elegans. EMBO REP. 2023 Jun 12} {CoIP} {Caenorhabditis elegans}
[IF={{ 14.903 }}] {Xin Yue. et al. Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1–BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy. ACTA PHARM SIN B. 2023 May;:} {WB} {Human}
[IF={{ 14.8 }}] {Li Weitao. et al. Adipose triglyceride lipase suppresses noncanonical inflammasome by hydrolyzing LPS. NAT CHEM BIOL. 2024 Feb;:1-9} {WB,IP} {Human,Mouse}
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA?either natural or formed from oligonucleotides?containing a high?affinity site for a DNA?binding protein provide a powerful tool for purification. The biotin/streptavidin purification system is based on the tight and essentially irreversible
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