Sample:
Lane 1: VP16-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug
Lane 2: VP16-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug
Primary: Anti-VP16 tag (bs-2111R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
- ￥1180 - 2800
- Bioss
- bs-2111R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2111R |
| 英文名称 | VP16 tag Rabbit pAb |
| 中文名称 | VP16 tag抗体 |
| 英文别名 | VP16_HHV1F; UL48; Tegument protein VP16; alpha trans-inducing protein; alpha-TIF; ICP25; Vmw65 |
| 产品应用 | WB=1:1000-5000, ELISA=1:1000-5000 Not yet tested in other applications. |
| 交叉反应 | Species independent |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from HSV-1 VP16 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Tags & Cell Markers > Fusion / Marker Proteins > VP16 Tag |
| 亚基 | Interacts with VP22. Interacts with gH (via C-terminus). Interacts with the virion host shutoff protein (vhs). Interacts with VP11/12. Associates with the VP16-induced complex; binding to host HCFC1 activates VP16 for association with the octamer motif-binding host protein POU2F1, to form a multiprotein-DNA complex responsible for activating transcription of the viral immediate early genes (By similarity). |
| 相似性 | Belongs to the herpesviridae tegument protein VP16 protein family. |
| 功能 | Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection (By similarity). May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | HSV evades the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking TAP or the transporter associated with antigen processing induced by the secretion of ICP-47 by HSV. In the host cell, TAP transports digested viral antigen epitope peptides from the cytosol to the endoplasmic reticulum, allowing these epitopes to be combined with MHC class I molecules and presented on the surface of the cell. Viral epitope presentation with MHC class I is a requirement for activation of cytotoxic T-lymphocytes (CTLs), the major effectors of the cell-mediated immune response against virally-infected cells. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:1000-5000} |
| {ELISA} | {1:1000-5000} |
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文献和实验相关实验
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
transformed with plasmid‐encoded proteins fused to affinity tag (T7 promoter‐based) (e.g., from the Center for Personalized Diagnostics of the Biodesign Institute at Arizona State University; 96‐well plates of bacterial glycerol stock
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VP16 tag Rabbit pAb(bs-2111R)-50ul/100ul/200ul
￥1180 - 2800
