产品封面图
文献支持

Galectin 3 Rabbit pAb(bs-20700

R)-50ul/100ul/200ul
收藏
  • ￥1180 - 2800
  • Bioss已认证
  • bs-20700R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
avatar
北京博奥森生物技术有限公司
品牌商
10 年钻石会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      产品信息以Bioss网站为准

    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-20700R
    英文名称Galectin 3 Rabbit pAb
    中文名称半乳糖凝集素3抗体
    英文别名LEG3_HUMAN; Galectin-3; LGALS3; Gal-3; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; CBP35; Galactose-specific lectin 3; Galactoside-binding protein ; GALBP; IgE-binding protein; L-31; Laminin-binding protein; Lectin L-29; Mac-2 antigen; MAC2; galectin 3; L31; GAL3; GALIG;
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Dog)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Galectin 3
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量29 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cell Biology > Cell Cycle > Cell differentiation

    Neuroscience > Cell Adhesion Proteins > Nuclear Proteins

    亚基Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.
    亚细胞定位Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface.
    组织特异性A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
    相似性Contains 1 galectin domain.
    功能Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料

    This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1ug/Test}

     

    产品细节图片1
    Sample:
    SW480(Human) Cell Lysate at 40 ug
    Primary: Anti-Galectin 3 (bs-20700R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 29 kD
    Observed band size: 29 kD
    产品细节图片2
    Sample:
    MCF-7 Cell (Human) Lysate at 40 ug
    NIH/3T3 Cell (Mouse) Lysate at 40 ug
    Primary: Anti-Galectin 3 (bs-20700R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 29 kD
    Observed band size: 29 kD
    产品细节图片3
    Blank control:A431.
    Primary Antibody (green line): Rabbit Anti-Galectin 3 antibody (bs-20700R)
    Dilution: 1μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-AF647
    Dilution: 1μg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    产品细节图片4
    Sample:
    Lane 1: Siha (Human) Cell Lysate at 30 ug
    Lane 2: U87MG (Human) Cell Lysate at 30 ug
    Lane 3: A431 (Human) Cell Lysate at 30 ug
    Lane 4: Lovo (Human) Cell Lysate at 30 ug
    Lane 5: MCF-7 (Human) Cell Lysate at 30 ug
    Lane 6: Hela (Human) Cell Lysate at 30 ug
    Primary:
    Anti- Galectin 3 (bs-20700R) at 1/1000 dilution
    Anti- beta-Actin (bs-0061R) at 1/2000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 26 kD
    Observed band size: 28 kD
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (bs-20700R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    该产品被引用文献

    [IF={{ preprint }}] {Yongcan Wu. et al.Ganduqing attenuates PM2.5-induced lung injury via regulating the pulmonary microbiota and metabolic proles.Research Square.} {IHC} {Rat}

    [IF={{ 7.793 }}] {Wang H et al. The effect of exposure time and concentration of airborne PM2. 5 on lung injury in mice: A transcriptome analysis. Redox Biol. 2019 Jul 2;26:101264.} {IHC} {Mouse}

    [IF={{ 7.793 }}] {Gao J et al. Metformin protects against PM2. 5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase α2. Redox Biol. 2019 Oct 19;28:101345.} {IHC} {Mouse}

    [IF={{ 7.129 }}] {Yongcan Wu. et al. Probiotics ameliorates pulmonary inflammation via modulating gut microbiota and rectifying Th17/Treg imbalance in a rat model of PM2.5 induced lung injury. ECOTOX ENVIRON SAFE. 2022 Oct;244:114060} {IHC} {Rat}

    [IF={{ 6.02 }}] {Wang H et al. AMPKα2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction.Free Radic Biol Med. 2018 Jun;121:202-214.} {Other} {Mouse,Human&Rat}

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Galectin 3 Rabbit pAb
    ￥600
    杭州戴格生物技术有限公司
    2025年07月09日询价
    Galectin-7 rabbit pAb
    ￥900
    上海贝博生物科技有限公司
    2025年07月11日询价
    Galectin-1/LGALS1 Rabbit pAb
    ￥1990
    上海圻明生物科技有限公司
    2025年07月10日询价
    Galectin 10 rabbit pAb
    询价
    ImmunoWay Biotechnology Company
    2025年07月06日询价
    Rabbit Galectin 3 Antibody/Rabbit Galectin 3 Antibody
    询价
    艾美捷科技有限公司
    2025年07月13日询价
    文献支持
    Galectin 3 Rabbit pAb(bs-20700R)-50ul/100ul/200ul
    ￥1180 - 2800