MAP2K6 | MEK6 (p-S202) Rabbit pAb(bs-3276R)-50ul/100ul/200ul产品图

MAP2K6 | MEK6 (p-S202) Rabbit

pAb(bs-3276R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-3276R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-3276R
    英文名称phospho-MEK6 (Ser202) Rabbit pAb
    中文名称磷酸化丝裂原活化蛋白激酶MKK6抗体
    英文别名MAP kinase kinase 6; MAPK/ERK kinase 6; MAPKK6; MEK6; Mitogen Activated Protein Kinase Kinase 6; MKK 6; MKK6; PRKMK6; SAPKK3; Dual specificity mitogen activated protein kinase kinase 6; MKK 6; Dual specificity mitogen activated protein kinase kinase 6; EC 2.7.12.2; MP2K6_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Mouse, Rat (Human)
    抗体来源Rabbit
    免疫原KLH conjugated synthesised phosphopeptide derived from human MEK6 around the phosphorylation site of Ser202
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量37 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Signal transduction > Protein phosphorylation > Serine/threonine kinases > MAPK pathway

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway

    亚基Interacts with Yersinia yopJ._x000D_
    亚细胞定位
    组织特异性Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas._x000D_
    翻译后修饰Weakly autophosphorylated. Phosphorylated by TAOK2.
    Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway._x000D_
    相似性Belongs to the enoyl-CoA hydratase/isomerase family._x000D_
    功能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: Rat heart tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-MAPKK6 Polyclonal Antibody, Unconjugated(bs-3276R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Sample:
    _x000D_ Muscle (Mouse) Lysate at 40 ug
    _x000D_ Primary: Anti- Phospho-MEK6 (Ser202) (bs-3276R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 37 kD
    _x000D_ Observed band size: 38 kD
    _x000D_
    产品细节图片3
    Sample:_x000D_ Lane 1: Mouse Large intestine tissue lysates_x000D_ Lane 2: Mouse Heart tissue lysates_x000D_ Lane 3: Mouse Muscle tissue lysates_x000D_ Primary: Anti-Phospho-MEK6 (Ser202) (bs-3276R) at 1/1000 dilution_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution_x000D_ Predicted band size: 37 kDa_x000D_ Observed band size: 42 kDa

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