Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SCN1A Polyclonal Antibody, Unconjugated(bs-20129R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-20129R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-20129R |
| 英文名称 | SCN1A Rabbit pAb |
| 中文名称 | SCN1A抗体 |
| 英文别名 | alpha; brain sodium channel type I; EIEE6; FEB3; FEB3A; FHM3; GEFS+2; GEFSP2; HBSC I; HBSCI; MIM 182390; NAC1; Nav 1.1; RBI; SCN1; Scn1a; SCN1A_HUMAN; SCN2A1; SMEI; sodium channel; Sodium channel protein brain I alpha subunit; Sodium channel protein brain I subunit alpha; Sodium channel protein type 1 subunit alpha; Sodium channel protein type I subunit alpha; Sodium channel voltage gated type 1 alpha subunit; Sodium channel voltage gated type I alpha polypeptide; type I; voltage gated; Voltage-gated sodium channel subunit alpha Nav1.1. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SCN1A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 229 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > Sodium Channels |
| 亚基 | The sodium channel consists of a large polypeptide and 2-3 smaller ones. This sequence represents a large polypeptide. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 相似性 | Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.1/SCN1A subfamily. Contains 1 IQ domain. |
| 功能 | Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SCN1A is a voltage-gated ion channel essential for the generation and propagation of action potentials, mainly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit, and two smaller auxiliary beta subunits. This gene encodes the large alpha subunit, and mutations in this gene have been associated with several epilepsy, convulsion and migraine disorders. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCN1A) Polyclonal Antibody, Unconjugated (bs-20129R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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SCN1A Rabbit pAb(bs-20129R)-50ul/100ul/200ul
￥1180 - 2800
