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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-17429R |
| 英文名称 | Histone H2B (acetyl K5) Rabbit pAb |
| 中文名称 | 乙酰化组蛋白H2B K5抗体 |
| 英文别名 | H2B 1A; H2B; H2B histone family; H2B2f; H2B2F_HUMAN; H2Ba; H2Bf; HIST2H2BF; histone H2B; histone H2B type 1; Histone H2B type 2-F; MGC131639. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Chicken, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised acetylpeptide derived from human Histone H2B around the acetylation site of Lys5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 14 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Histones > H2B > Acetylated |
| 亚细胞定位 | Nucleus. Chromosome. |
| 翻译后修饰 | Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
Phosphorylation at Ser-37 (H2BS36ph) by AMPK in response to stress promotes transcription (By similarity). Phosphorylated on Ser-15 (H2BS14ph) by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination. GlcNAcylation at Ser-113 promotes monoubiquitination of Lys-121. It fluctuates in response to extracellular glucose, and associates with transcribed genes. |
| 相似性 | Belongs to the histone H2B family. |
| 功能 | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
-diphosphate (ADP)-ribosylation, methylation, ubiquitination, and acetylation (1 ,2 ). Histone acetylation involves the transfer of an acetyl group from acetyl-coenzyme A (CoA) to the ε-amino group of lysine side chains within the substrate by histone acetyl
IHC IC2575Acetyl-Histone H2B (Lys12) AntibodyRabbitW IHC IC2571Acetyl-Histone H2B (Lys20) AntibodyRabbitW IP IHC IC2574Acetyl-Histone H2B (Lys5) AntibodyRabbitW IP IHC IC9675Acetyl-Histone H3 (Lys18) AntibodyRabbitW IHC IC9674Acetyl-Histone H3 (Lys23
结果分析的重要步骤和Trouble shooting的依据，因为非特异性的免疫沉淀难以避免。 一般的设置方法 1 未经免疫 沉淀的超声处理后样本( INPUT)。 2 加特异抗体来源动物的正常血清或无关抗体(MOCK)。 3 阳性对照引物的应用。 4 选择不与目的蛋白质结合的基因组 区域作对照。 如 阳性对照抗体 (Anti-acetyl-Histone H3) * 阴性对照抗体 (Normal Rabbit
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