Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SALL4 Polyclonal Antibody, Unconjugated(bs-12204R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-12204R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12204R |
| 英文名称 | SALL4 Rabbit pAb |
| 中文名称 | 锌指转录蛋白Sall4抗体 |
| 英文别名 | AA407717; ZNF797; AL022809; AW536104; C330011P20Rik; C78083; C78563; Sal like 4(Drosophila); Sal like 4; Sal like Protein 4; Sal-like protein 4; Sall4; SALL4_HUMAN; Tex20; Zinc finger protein 797; Zinc finger protein SALL4. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SALL4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 112 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Embryogenesis > Embryonic stem cells > Surface molecules Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Stem Cells > Embryonic Stem Cells > Intracellular Stem Cells > Signaling Pathways > Wnt > Nuclear |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in testis. |
| 相似性 | Belongs to the sal C2H2-type zinc-finger protein family. Contains 7 C2H2-type zinc fingers. |
| 功能 | Probable transcription factor. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Sall4 is mammalian homologs of the Drosophila region-specific homeotic gene spalt, which encodes a zinc finger-containing transcription regulator. Drosophila spalt is an essential genetic component required for the specification of posterior head and anterior tail as opposed to trunk. Sall3 is expressed at 24 weeks of gestation in several regions of the human fetal brain including neurons of the hippocampus formation and of mediodorsal and ventrolateral thalamic nuclei, Purkinje cells of the cerebellum, and a subset of neurons in the brainstem. Sall4 expression in early mouse embryos is gradually confined to the head region and the primitive streak, followed by prominent expression in the developing midbrain, branchial arches, limbs and genital papilla. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-SALL4 (bs-12204R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 118 kDa
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human Jurkat cell lysates
Primary: Anti-SALL4 (bs-12204R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 118 kDa
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文献和实验该产品被引用文献
[IF={{ 2.775 }}] {Ru Yu. et al. ATF6 deficiency damages the development of spermatogenesis in male Atf6 knockout mice. 2021 Dec 13} {IHC} {Mouse}
[IF={{ 2.4 }}] {Zhao Xuan. et al. CircSFMBT2 Plays an Oncogenic Role in Lung Adenocarcinoma Depending on the miR-1305/SALL4 Axis. BIOCHEM GENET. 2023 Dec;:1-19} {WB} {Human}
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SALL4 Rabbit pAb(bs-12204R)-50ul/100ul/200ul
￥1180 - 2800
