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phospho-PDPK1 (Tyr9) Rabbit pA

b(bs-5543R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-5543R
  • 2025年10月24日
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-5543R
    英文名称phospho-PDPK1 (Tyr9) Rabbit pAb
    中文名称磷酸化3磷酸肌醇依赖性蛋白激酶1抗体
    英文别名PDPK1(phospho Y9); p-PDPK1(phospho Y9); PDPK1(phospho Tyr9); 3 phosphoinositide dependent protein kinase 1; hPDK 1; hPDK1; MGC20087 MGC35290; PDK 1; PDPK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PkB like; PRO0461; PDPK1_HUMAN.
    产品应用WB=1:500-2000, ICC/IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human PDK1 around the phosphorylation site of Tyr9
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量61 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Assembly

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases

    亚基Interacts with NPRL2.
    亚细胞定位Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
    组织特异性Appears to be expressed ubiquitously.
    翻译后修饰Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
    相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    功能Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {ICC/IF}{1:50-200}

     

    产品细节图片1
    Sample:
    Liver (Mouse) Lysate at 40 ug
    Heart (Mouse) Lysate at 40 ug
    Primary: Anti-Phospho-PDPK1(Tyr9) (bs-5543R) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 61 kD
    Observed band size: 51 kD
    产品细节图片2
    HepG2 cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-PDPK1 (Tyr9)) polyclonal Antibody, Unconjugated (bs-5543R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

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