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- bs-1529R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1529R |
| 英文名称 | CD46 Rabbit pAb |
| 中文名称 | 膜辅蛋白抗体 |
| 英文别名 | CD46 antigen; CD46 molecule; CD46 molecule complement regulatory protein; Complement membrane cofactor protein; MCP; Measles virus receptor; Membrane cofactor protein; Membrane cofactor protein precursor; MIC 10; MIC10; TLX; TRA2.10; Trophoblast leucocyte common antigen; Trophoblast lymphocyte cross reactive antigen; Antigen identified by monoclonal antibody TRA 2 10; MCP_HUMAN; membrane cofactor protein isoform 1 precursor. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD46 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 43 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Cell Type Markers > CD > Non-lineage Immunology > Innate Immunity > Complement > Regulatory |
| 亚基 | Interacts with C3b and C4b. Binds to Measles virus H protein, to Human herpesvirus 6 GH protein and to human adenovirus B/D PIV/fiber protein, and acts as a receptor for these viruses. Binds to Streptococcus pyogenes M protein and to type IV pili from Neisseria, and may act as a receptor for these pathogenic bacteria. |
| 亚细胞定位 | Isoform 1: Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane; Single-pass type I membrane protein. Note=Inner acrosomal membrane of spermatozoa. _x000D_ Isoform 2: Secreted (Probable). |
| 组织特异性 | Present only in testis (at protein level). |
| 翻译后修饰 | May be O-glycosylated (By similarity). _x000D_ N-glycosylated. |
| 相似性 | Contains 4 Sushi (CCP/SCR) domains. |
| 功能 | May be involved in the fusion of the spermatozoa with the oocyte during fertilization. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in the regulation of the complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg /test} |
Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD46) Polyclonal Antibody, Unconjugated (bs-1529R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: 293T(blue).
_x000D_ Primary Antibody:Rabbit Anti-CD46 antibody(bs-1529R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green);
_x000D_ Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions );
_x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ protocol
_x000D_ The cells were washed twice with phosphate-buffered saline (PBS).The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-1529R, 1μg/1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice.Acquisition of 20,000 events was performed. _x000D_
_x000D_ Primary Antibody:Rabbit Anti-CD46 antibody(bs-1529R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA(green);
_x000D_ Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions );
_x000D_ Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ protocol
_x000D_ The cells were washed twice with phosphate-buffered saline (PBS).The cells were then incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (bs-1529R, 1μg/1x10^6 cells) for 30 min on ice. The secondary antibody used was Goat Anti-rabbit IgG/PE antibody at 1/200 dilution for 30 min on ice.Acquisition of 20,000 events was performed. _x000D_
Blank control: U937 (blue).
Primary Antibody: Rabbit Anti- CD46 antibody(bs-1529R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde (10 min). Primary antibody (bs-1529R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
Primary Antibody: Rabbit Anti- CD46 antibody(bs-1529R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde (10 min). Primary antibody (bs-1529R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
Sample:
_x000D_ Lane 1: Mouse Placenta tissue lysates
_x000D_ Lane 2: Mouse Lung tissue lysates
_x000D_ Lane 3: Human K562 cell lysates
_x000D_ Lane 4: Human HeLa cell lysates
_x000D_ Lane 5: Human MOLT4 cell lysates
_x000D_ Lane 6: Human MCF-7 cell lysates
_x000D_ Lane 7: Human Daudi cell lysates
_x000D_ Primary: Anti-CD46 (bs-1529R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 43 kDa
_x000D_ Observed band size: 52 kDa
_x000D_ Lane 1: Mouse Placenta tissue lysates
_x000D_ Lane 2: Mouse Lung tissue lysates
_x000D_ Lane 3: Human K562 cell lysates
_x000D_ Lane 4: Human HeLa cell lysates
_x000D_ Lane 5: Human MOLT4 cell lysates
_x000D_ Lane 6: Human MCF-7 cell lysates
_x000D_ Lane 7: Human Daudi cell lysates
_x000D_ Primary: Anti-CD46 (bs-1529R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 43 kDa
_x000D_ Observed band size: 52 kDa
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文献和实验该产品被引用文献
[IF=2.6] Masahisa Hemmi. et al. Neutralizing monoclonal antibodies improve biodistribution of intravenously administered oncolytic adenovirus in human CD46-transgenic mice. PLOS ONE. 2025 Jun;20(6):e0326857 WB Mouse
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CD46 | MCP Rabbit pAb(bs-1529R)-50ul/100ul/200ul
￥1180 - 2800
