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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
fragment exo- can be used for adding "A" bases. 42. Perform linker ligation. Combine: 10 µL of DNA (300 ng) 9.9 µL of H2 O 2.5 µL of T4 DNA ligase buffer (10
Electrophoretic Mobility Shift Assay(EMSA)
, bottom and side with binder clips• Lie on a slight angle (can use microfuge tube rack) Native PAGE Preparation:Add in this order63.25ml dH2O3.75ml 10x TBE7.5ml 40% 37:5:1 acrylamide0.5ml 10% APS50μl TEMED0.5x TBE: 50ml 10x TBE,950ml dH2O Gel Preparation Before Sample
Electrophoretic Mobility Shift Assay (EMSA)
,bottom and side with binder clips • Lie on a slight angle (can use microfuge tube rack) Native PAGE Preparation: Add in this order 63.25ml dH2O 3.75ml 10x TBE 7.5ml 40% 37:5:1 acrylamide 0.5ml 10% APS 50μl TEMED 0.5x TBE: 50ml 10x TBE 950ml dH2O Gel Preparation
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