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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
pJA51
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: pJA51
其他名称:
质粒大小(bp): 7555
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
| Notes: |
Shuttle vector to facilitate construction of gene disruptions in E. coli. The gene of interest is cleaved at an appropriate site, made flush, and ligated to the SmaI fragment from this plasmid that contains kanR and the yeast selectable marker. (personal communication) The order of the major features in this plasmid is:LEU2 – kanR – EcoRI/MCS/HindIII – f1 ori – ampR – pMB1 ori. (personal communication) The appropriate product is selected for by kanR in E.coli. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI–5.3, 2.3; PstI–4.9, 2.8; HindIII–3.8, 3.8; BglII–6.5, 1.1; BamHI–7.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli.
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质粒图谱:
质粒序列:
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文献和实验CD51 分子 CD51 常用单克隆抗体或代号: 13C2,23C6,NK1- M7;(VNRa 链,integrin a V) 主要表达细胞: Pt,En,Meg [Pt] 分子质量(kDa)和结构: gp150,与CD61组成二聚体 功 能: 粘附VN、FN和vWF CD51 Aka vitronectin receptor-alpha chain; beta chain
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
一、用 51 Cr 铬酸钠标记靶细胞 短期标记法 1 .用 RPMI-1640 培养液洗涤 107 靶细胞,去上清液。用 0.5ml 不含碳酸氢钠的完全培养液悬浮细胞。加入 100 μ Ci ( 3.7MBq ) 51 Cr 铬酸钠, 37 ℃水浴中标记 1 小时,每 5 ~ 10 分钟摇晃一次,混匀细胞。 2 .如上用 RPMI-1640 培养液洗涤
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