Restriction digests of the clone give the following sizes (kb): EcoRI–4.4; HindIII–4.4; BamHI–4.4; SmaI–4.4; PvuI–3.0, 1.5. (ATCC staff) Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and SiCMV IE94 promoter (-990 to +30, NF1 binding cluster, enhancer/promoter region). (personal communication) A mammalian expression vector for expression of a sequence cloned into the polylinker site under the control of the SiCMV IE94 promoter. (ATCC staff) Medium is 1315 1065 plus ampicillin (100 ug/ul). Hosts: E.coli HB101, vertebrate cells, E.coli, E.coli DH5.