pETG-30A

名称	编号	规格	价格	手册
pETG-30A(pETG30A)

质粒编号:
质粒名称: pETG-30A
其他名称:
质粒大小（bp）: 7784
抗性: Ampicillin
质粒类型: Other, GST destination vector
是否病毒: Unspecified
启动子: T7
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签: GST, His
5’测序引物: pGEX-5
GenBank号:
其他属性:

Selectable Marker:	Tet

质粒图谱:

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
attR1	190	290
attR2	290	190
ccdB	636	331
CAT/CamR	1637	978
attR1	1870	1746
attR2	1746	1846
6xHIS	2575	2558
T7_transl_en_RBS	2605	2589
lacO	2650	2623
T7_promoter	2668	2650
tet (300 – 563)	2700	2963
pBRrevBam_primer	2771	2752
lacI	3046	4137
tet (576 – 851)	4196	4471
ROP	4946	5137
pGEX_3_primer	5153	5131
pBR322_origin	6171	5552
Ampicillin	7186	6326
AmpR_promoter	7256	7228

ORF	Start	End
ORF frame 1	1637	978
ORF frame 2	2581	1835
ORF frame 3	2805	3305
ORF frame 1	3178	4137
ORF frame 2	7186	6326

Enzyme Name	Cut
XhoI	158
SalI	291
SmaI	552
XmaI	550
NcoI	1118
EcoRI	1419
NotI	1739
EagI	1739
KpnI	1878
BstBI	2139
SpeI	2549
XbaI	2617
BglII	2683
ApaI	3616
EcoRV	3855
HpaI	3911

质粒序列:

1	ATCCGGATAT	AGTTCCTCCT	TTCAGCAAAA	AACCCCTCAA	GACCCGTTTA	50
51	GAGGCCCCAA	GGGGTTATGC	TAGTTATTGC	TCAGCGGTGG	CAGCAGCCAA	100
101	CTCAGCTTCC	TTTCGGGCTT	TGTTAGCAGC	CGGATCTCAG	TGGTGGTGGT	150
151	GGTGGTGCTC	GAGTCACCAC	TTTGTACAAG	AAAGCTGAAC	GAGAAACGTA	200
201	AAATGATATA	AATATCAATA	TATTAAATTA	GATTTTGCAT	AAAAAACAGA	250
251	CTACATAATA	CTGTAAAACA	CAACATATCC	AGTCACTATG	GTCGACCTGC	300
301	AGACTGGCTG	TGTATAAGGG	AGCCTGACAT	TTATATTCCC	CAGAACATCA	350
351	GGTTAATGGC	GTTTTTGATG	TCATTTTCGC	GGTGGCTGAG	ATCAGCCACT	400
401	TCTTCCCCGA	TAACGGAGAC	CGGCACACTG	GCCATATCGG	TGGTCATCAT	450
451	GCGCCAGCTT	TCATCCCCGA	TATGCACCAC	CGGGTAAAGT	TCACGGGAGA	500
501	CTTTATCTGA	CAGCAGACGT	GCACTGGCCA	GGGGGATCAC	CATCCGTCGC	550
551	CCGGGCGTGT	CAATAATATC	ACTCTGTACA	TCCACAAACA	GACGATAACG	600
601	GCTCTCTCTT	TTATAGGTGT	AAACCTTAAA	CTGCATTTCA	CCAGTCCCTG	650
651	TTCTCGTCAG	CAAAAGAGCC	GTTCATTTCA	ATAAACCGGG	CGACCTCAGC	700
701	CATCCCTTCC	TGATTTTCCG	CTTTCCAGCG	TTCGGCACGC	AGACGACGGG	750
751	CTTCATTCTG	CATGGTTGTG	CTTACCAGAC	CGGAGATATT	GACATCATAT	800
801	ATGCCTTGAG	CAACTGATAG	CTGTCGCTGT	CAACTGTCAC	TGTAATACGC	850
851	TGCTTCATAG	CACACCTCTT	TTTGACATAC	TTCGGGTATA	CATATCAGTA	900
901	TATATTCTTA	TACCGCAAAA	ATCAGCGCGC	AAATACGCAT	ACTGTTATCT	950
951	GGCTTTTAGT	AAGCCGGATC	CACGCGTTTA	CGCCCCGCCC	TGCCACTCAT	1000
1001	CGCAGTACTG	TTGTAATTCA	TTAAGCATTC	TGCCGACATG	GAAGCCATCA	1050
1051	CAGACGGCAT	GATGAACCTG	AATCGCCAGC	GGCATCAGCA	CCTTGTCGCC	1100
1101	TTGCGTATAA	TATTTGCCCA	TGGTGAAAAC	GGGGGCGAAG	AAGTTGTCCA	1150
1151	TATTGGCCAC	GTTTAAATCA	AAACTGGTGA	AACTCACCCA	GGGATTGGCT	1200
1201	GAGACGAAAA	ACATATTCTC	AATAAACCCT	TTAGGGAAAT	AGGCCAGGTT	1250
1251	TTCACCGTAA	CACGCCACAT	CTTGCGAATA	TATGTGTAGA	AACTGCCGGA	1300
1301	AATCGTCGTG	GTATTCACTC	CAGAGCGATG	AAAACGTTTC	AGTTTGCTCA	1350
1351	TGGAAAACGG	TGTAACAAGG	GTGAACACTA	TCCCATATCA	CCAGCTCACC	1400
1401	GTCTTTCATT	GCCATACGGA	ATTCCGGATG	AGCATTCATC	AGGCGGGCAA	1450
1451	GAATGTGAAT	AAAGGCCGGA	TAAAACTTGT	GCTTATTTTT	CTTTACGGTC	1500
1501	TTTAAAAAGG	CCGTAATATC	CAGCTGAACG	GTCTGGTTAT	AGGTACATTG	1550
1551	AGCAACTGAC	TGAAATGCCT	CAAAATGTTC	TTTACGATGC	CATTGGGATA	1600
1601	TATCAACGGT	GGTATATCCA	GTGATTTTTT	TCTCCATTTT	AGCTTCCTTA	1650
1651	GCTCCTGAAA	ATCTCGCCGG	ATCCTAACTC	AAAATCCACA	CATTATACGA	1700
1701	GCCGGAAGCA	TAAAGTGTAA	AGCCTGGGGT	GCCTAATGCG	GCCGCCATAG	1750
1751	TGACTGGATA	TGTTGTGTTT	TACAGTATTA	TGTAGTCTGT	TTTTTATGCA	1800
1801	AAATCTAATT	TAATATATTG	ATATTTATAT	CATTTTACGT	TTCTCGTTCA	1850
1851	GCTTTTTTGT	ACAAACTTGT	CATGGTACCA	TCCGATTTTG	GAGGATGGTC	1900
1901	GCCACCACCA	AACGTGGCTT	GCCAGCCCTG	CAAAGGCCAT	GCTATATACT	1950
1951	TGCTGGATTT	CAAGTACTTA	TCAATTTGTG	GGATAGCTTC	AATACGTTTT	2000
2001	TTAAAACAAA	CTAATTTTGG	GAACGCATCC	AGGCACATTG	GGTCCATGTA	2050
2051	TAAAACAACA	TCAAGAGCGT	CATACAACAT	GAAGTCAGGA	TGGGTTACAT	2100
2101	GATCACCATT	TAAATATGTT	TTATGACATA	AACGATCTTC	GAACATTTTC	2150
2151	AGCATTTCAG	GTAGCTTGCT	AAGAAAATCA	ACTTTGAGAG	TTTCAAAGTC	2200
2201	TTTACTATAT	GCAATTCTCG	AAACACCGTA	TCTAATATCC	AAAACCGCTC	2250
2251	CTTCAAGCAT	TGAAATCTCT	GCACGCTCTT	TTGGACAACC	ACCCAACATG	2300
2301	TTGTGCTTGT	CAGCTATATA	ACGTATGATG	GCCATAGACT	GTGTTAATTT	2350
2351	AACATCACCA	TCAATATAAT	AAGGAAGATT	GGGAAACTCC	AAACCCAATT	2400
2401	CAAACTTTTT	GTTTCGCCAT	TTATCACCTT	CATCGCGCTC	ATACAAATGC	2450
2451	TCTTCATATT	TTTCTTCAAG	ATATTCCAAA	AGAAGTCGAG	TGGGTTGCAC	2500
2501	AAGGCCCTTA	ATTTTCCAAT	AACCTAGTAT	AGGGGACATG	GAATTGCTAC	2550
2551	TAGTGTTGTG	ATGGTGATGG	TGATGTTTCA	TGGTATATCT	CCTTCTTAAA	2600
2601	GTTAAACAAA	ATTATTTCTA	GAGGGGAATT	GTTATCCGCT	CACAATTCCC	2650
2651	CTATAGTGAG	TCGTATTAAT	TTCGCGGGAT	CGAGATCTCG	ATCCTCTACG	2700
2701	CCGGACGCAT	CGTGGCCGGC	ATCACCGGCG	CCACAGGTGC	GGTTGCTGGC	2750
2751	GCCTATATCG	CCGACATCAC	CGATGGGGAA	GATCGGGCTC	GCCACTTCGG	2800
2801	GCTCATGAGC	GCTTGTTTCG	GCGTGGGTAT	GGTGGCAGGC	CCCGTGGCCG	2850
2851	GGGGACTGTT	GGGCGCCATC	TCCTTGCATG	CACCATTCCT	TGCGGCGGCG	2900
2901	GTGCTCAACG	GCCTCAACCT	ACTACTGGGC	TGCTTCCTAA	TGCAGGAGTC	2950
2951	GCATAAGGGA	GAGCGTCGAG	ATCCCGGACA	CCATCGAATG	GCGCAAAACC	3000
3001	TTTCGCGGTA	TGGCATGATA	GCGCCCGGAA	GAGAGTCAAT	TCAGGGTGGT	3050
3051	GAATGTGAAA	CCAGTAACGT	TATACGATGT	CGCAGAGTAT	GCCGGTGTCT	3100
3101	CTTATCAGAC	CGTTTCCCGC	GTGGTGAACC	AGGCCAGCCA	CGTTTCTGCG	3150
3151	AAAACGCGGG	AAAAAGTGGA	AGCGGCGATG	GCGGAGCTGA	ATTACATTCC	3200
3201	CAACCGCGTG	GCACAACAAC	TGGCGGGCAA	ACAGTCGTTG	CTGATTGGCG	3250
3251	TTGCCACCTC	CAGTCTGGCC	CTGCACGCGC	CGTCGCAAAT	TGTCGCGGCG	3300
3301	ATTAAATCTC	GCGCCGATCA	ACTGGGTGCC	AGCGTGGTGG	TGTCGATGGT	3350
3351	AGAACGAAGC	GGCGTCGAAG	CCTGTAAAGC	GGCGGTGCAC	AATCTTCTCG	3400
3401	CGCAACGCGT	CAGTGGGCTG	ATCATTAACT	ATCCGCTGGA	TGACCAGGAT	3450
3451	GCCATTGCTG	TGGAAGCTGC	CTGCACTAAT	GTTCCGGCGT	TATTTCTTGA	3500
3501	TGTCTCTGAC	CAGACACCCA	TCAACAGTAT	TATTTTCTCC	CATGAAGACG	3550
3551	GTACGCGACT	GGGCGTGGAG	CATCTGGTCG	CATTGGGTCA	CCAGCAAATC	3600
3601	GCGCTGTTAG	CGGGCCCATT	AAGTTCTGTC	TCGGCGCGTC	TGCGTCTGGC	3650
3651	TGGCTGGCAT	AAATATCTCA	CTCGCAATCA	AATTCAGCCG	ATAGCGGAAC	3700
3701	GGGAAGGCGA	CTGGAGTGCC	ATGTCCGGTT	TTCAACAAAC	CATGCAAATG	3750
3751	CTGAATGAGG	GCATCGTTCC	CACTGCGATG	CTGGTTGCCA	ACGATCAGAT	3800
3801	GGCGCTGGGC	GCAATGCGCG	CCATTACCGA	GTCCGGGCTG	CGCGTTGGTG	3850
3851	CGGATATCTC	GGTAGTGGGA	TACGACGATA	CCGAAGACAG	CTCATGTTAT	3900
3901	ATCCCGCCGT	TAACCACCAT	CAAACAGGAT	TTTCGCCTGC	TGGGGCAAAC	3950
3951	CAGCGTGGAC	CGCTTGCTGC	AACTCTCTCA	GGGCCAGGCG	GTGAAGGGCA	4000
4001	ATCAGCTGTT	GCCCGTCTCA	CTGGTGAAAA	GAAAAACCAC	CCTGGCGCCC	4050
4051	AATACGCAAA	CCGCCTCTCC	CCGCGCGTTG	GCCGATTCAT	TAATGCAGCT	4100
4101	GGCACGACAG	GTTTCCCGAC	TGGAAAGCGG	GCAGTGAGCG	CAACGCAATT	4150
4151	AATGTAAGTT	AGCTCACTCA	TTAGGCACCG	GGATCTCGAC	CGATGCCCTT	4200
4201	GAGAGCCTTC	AACCCAGTCA	GCTCCTTCCG	GTGGGCGCGG	GGCATGACTA	4250
4251	TCGTCGCCGC	ACTTATGACT	GTCTTCTTTA	TCATGCAACT	CGTAGGACAG	4300
4301	GTGCCGGCAG	CGCTCTGGGT	CATTTTCGGC	GAGGACCGCT	TTCGCTGGAG	4350
4351	CGCGACGATG	ATCGGCCTGT	CGCTTGCGGT	ATTCGGAATC	TTGCACGCCC	4400
4401	TCGCTCAAGC	CTTCGTCACT	GGTCCCGCCA	CCAAACGTTT	CGGCGAGAAG	4450
4451	CAGGCCATTA	TCGCCGGCAT	GGCGGCCCCA	CGGGTGCGCA	TGATCGTGCT	4500
4501	CCTGTCGTTG	AGGACCCGGC	TAGGCTGGCG	GGGTTGCCTT	ACTGGTTAGC	4550
4551	AGAATGAATC	ACCGATACGC	GAGCGAACGT	GAAGCGACTG	CTGCTGCAAA	4600
4601	ACGTCTGCGA	CCTGAGCAAC	AACATGAATG	GTCTTCGGTT	TCCGTGTTTC	4650
4651	GTAAAGTCTG	GAAACGCGGA	AGTCAGCGCC	CTGCACCATT	ATGTTCCGGA	4700
4701	TCTGCATCGC	AGGATGCTGC	TGGCTACCCT	GTGGAACACC	TACATCTGTA	4750
4751	TTAACGAAGC	GCTGGCATTG	ACCCTGAGTG	ATTTTTCTCT	GGTCCCGCCG	4800
4801	CATCCATACC	GCCAGTTGTT	TACCCTCACA	ACGTTCCAGT	AACCGGGCAT	4850
4851	GTTCATCATC	AGTAACCCGT	ATCGTGAGCA	TCCTCTCTCG	TTTCATCGGT	4900
4901	ATCATTACCC	CCATGAACAG	AAATCCCCCT	TACACGGAGG	CATCAGTGAC	4950
4951	CAAACAGGAA	AAAACCGCCC	TTAACATGGC	CCGCTTTATC	AGAAGCCAGA	5000
5001	CATTAACGCT	TCTGGAGAAA	CTCAACGAGC	TGGACGCGGA	TGAACAGGCA	5050
5051	GACATCTGTG	AATCGCTTCA	CGACCACGCT	GATGAGCTTT	ACCGCAGCTG	5100
5101	CCTCGCGCGT	TTCGGTGATG	ACGGTGAAAA	CCTCTGACAC	ATGCAGCTCC	5150
5151	CGGAGACGGT	CACAGCTTGT	CTGTAAGCGG	ATGCCGGGAG	CAGACAAGCC	5200
5201	CGTCAGGGCG	CGTCAGCGGG	TGTTGGCGGG	TGTCGGGGCG	CAGCCATGAC	5250
5251	CCAGTCACGT	AGCGATAGCG	GAGTGTATAC	TGGCTTAACT	ATGCGGCATC	5300
5301	AGAGCAGATT	GTACTGAGAG	TGCACCATAT	ATGCGGTGTG	AAATACCGCA	5350
5351	CAGATGCGTA	AGGAGAAAAT	ACCGCATCAG	GCGCTCTTCC	GCTTCCTCGC	5400
5401	TCACTGACTC	GCTGCGCTCG	GTCGTTCGGC	TGCGGCGAGC	GGTATCAGCT	5450
5451	CACTCAAAGG	CGGTAATACG	GTTATCCACA	GAATCAGGGG	ATAACGCAGG	5500
5501	AAAGAACATG	TGAGCAAAAG	GCCAGCAAAA	GGCCAGGAAC	CGTAAAAAGG	5550
5551	CCGCGTTGCT	GGCGTTTTTC	CATAGGCTCC	GCCCCCCTGA	CGAGCATCAC	5600
5601	AAAAATCGAC	GCTCAAGTCA	GAGGTGGCGA	AACCCGACAG	GACTATAAAG	5650
5651	ATACCAGGCG	TTTCCCCCTG	GAAGCTCCCT	CGTGCGCTCT	CCTGTTCCGA	5700
5701	CCCTGCCGCT	TACCGGATAC	CTGTCCGCCT	TTCTCCCTTC	GGGAAGCGTG	5750
5751	GCGCTTTCTC	ATAGCTCACG	CTGTAGGTAT	CTCAGTTCGG	TGTAGGTCGT	5800
5801	TCGCTCCAAG	CTGGGCTGTG	TGCACGAACC	CCCCGTTCAG	CCCGACCGCT	5850
5851	GCGCCTTATC	CGGTAACTAT	CGTCTTGAGT	CCAACCCGGT	AAGACACGAC	5900
5901	TTATCGCCAC	TGGCAGCAGC	CACTGGTAAC	AGGATTAGCA	GAGCGAGGTA	5950
5951	TGTAGGCGGT	GCTACAGAGT	TCTTGAAGTG	GTGGCCTAAC	TACGGCTACA	6000
6001	CTAGAAGGAC	AGTATTTGGT	ATCTGCGCTC	TGCTGAAGCC	AGTTACCTTC	6050
6051	GGAAAAAGAG	TTGGTAGCTC	TTGATCCGGC	AAACAAACCA	CCGCTGGTAG	6100
6101	CGGTGGTTTT	TTTGTTTGCA	AGCAGCAGAT	TACGCGCAGA	AAAAAAGGAT	6150
6151	CTCAAGAAGA	TCCTTTGATC	TTTTCTACGG	GGTCTGACGC	TCAGTGGAAC	6200
6201	GAAAACTCAC	GTTAAGGGAT	TTTGGTCATG	AGATTATCAA	AAAGGATCTT	6250
6251	CACCTAGATC	CTTTTAAATT	AAAAATGAAG	TTTTAAATCA	ATCTAAAGTA	6300
6301	TATATGAGTA	AACTTGGTCT	GACAGTTACC	AATGCTTAAT	CAGTGAGGCA	6350
6351	CCTATCTCAG	CGATCTGTCT	ATTTCGTTCA	TCCATAGTTG	CCTGACTCCC	6400
6401	CGTCGTGTAG	ATAACTACGA	TACGGGAGGG	CTTACCATCT	GGCCCCAGTG	6450
6451	CTGCAATGAT	ACCGCGAGAC	CCACGCTCAC	CGGCTCCAGA	TTTATCAGCA	6500
6501	ATAAACCAGC	CAGCCGGAAG	GGCCGAGCGC	AGAAGTGGTC	CTGCAACTTT	6550
6551	ATCCGCCTCC	ATCCAGTCTA	TTAATTGTTG	CCGGGAAGCT	AGAGTAAGTA	6600
6601	GTTCGCCAGT	TAATAGTTTG	CGCAACGTTG	TTGCCATTGC	TGCAGGCATC	6650
6651	GTGGTGTCAC	GCTCGTCGTT	TGGTATGGCT	TCATTCAGCT	CCGGTTCCCA	6700
6701	ACGATCAAGG	CGAGTTACAT	GATCCCCCAT	GTTGTGCAAA	AAAGCGGTTA	6750
6751	GCTCCTTCGG	TCCTCCGATC	GTTGTCAGAA	GTAAGTTGGC	CGCAGTGTTA	6800
6801	TCACTCATGG	TTATGGCAGC	ACTGCATAAT	TCTCTTACTG	TCATGCCATC	6850
6851	CGTAAGATGC	TTTTCTGTGA	CTGGTGAGTA	CTCAACCAAG	TCATTCTGAG	6900
6901	AATAGTGTAT	GCGGCGACCG	AGTTGCTCTT	GCCCGGCGTC	AATACGGGAT	6950
6951	AATACCGCGC	CACATAGCAG	AACTTTAAAA	GTGCTCATCA	TTGGAAAACG	7000
7001	TTCTTCGGGG	CGAAAACTCT	CAAGGATCTT	ACCGCTGTTG	AGATCCAGTT	7050
7051	CGATGTAACC	CACTCGTGCA	CCCAACTGAT	CTTCAGCATC	TTTTACTTTC	7100
7101	ACCAGCGTTT	CTGGGTGAGC	AAAAACAGGA	AGGCAAAATG	CCGCAAAAAA	7150
7151	GGGAATAAGG	GCGACACGGA	AATGTTGAAT	ACTCATACTC	TTCCTTTTTC	7200
7201	AATATTATTG	AAGCATTTAT	CAGGGTTATT	GTCTCATGAG	CGGATACATA	7250
7251	TTTGAATGTA	TTTAGAAAAA	TAAACAAATA	GGGGTTCCGC	GCACATTTCC	7300
7301	CCGAAAAGTG	CCACCTGAAA	TTGTAAACGT	TAATATTTTG	TTAAAATTCG	7350
7351	CGTTAAATTT	TTGTTAAATC	AGCTCATTTT	TTAACCAATA	GGCCGAAATC	7400
7401	GGCAAAATCC	CTTATAAATC	AAAAGAATAG	ACCGAGATAG	GGTTGAGTGT	7450
7451	TGTTCCAGTT	TGGAACAAGA	GTCCACTATT	AAAGAACGTG	GACTCCAACG	7500
7501	TCAAAGGGCG	AAAAACCGTC	TATCAGGGCG	ATGGCCCACT	ACGTGAACCA	7550
7551	TCACCCTAAT	CAAGTTTTTT	GGGGTCGAGG	TGCCGTAAAG	CACTAAATCG	7600
7601	GAACCCTAAA	GGGAGCCCCC	GATTTAGAGC	TTGACGGGGA	AAGCCGGCGA	7650
7651	ACGTGGCGAG	AAAGGAAGGG	AAGAAAGCGA	AAGGAGCGGG	CGCTAGGGCG	7700
7701	CTGGCAAGTG	TAGCGGTCAC	GCTGCGCGTA	ACCACCACAC	CCGCCGCGCT	7750
7751	TAATGCGCCG	CTACAGGGCG	CGTCCCATTC	GCCA	7784