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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
pET151 Directional TOPO
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: pET151 Directional TOPO
其他名称:
质粒大小(bp): 5760
抗性: Ampicillin
质粒类型: Bacterial Expression
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:
质粒序列:
| 1 | CAAGGAGATG | GCGCCCAACA | GTCCCCCGGC | CACGGGGCCT | GCCACCATAC | 50 |
| 51 | CCACGCCGAA | ACAAGCGCTC | ATGAGCCCGA | AGTGGCGAGC | CCGATCTTCC | 100 |
| 101 | CCATCGGTGA | TGTCGGCGAT | ATAGGCGCCA | GCAACCGCAC | CTGTGGCGCC | 150 |
| 151 | GGTGATGCCG | GCCACGATGC | GTCCGGCGTA | GAGGATCGAG | ATCTCGATCC | 200 |
| 201 | CGCGAAATTA | ATACGACTCA | CTATAGGGGA | ATTGTGAGCG | GATAACAATT | 250 |
| 251 | CCCCTCTAGA | AATAATTTTG | TTTAACTTTA | AGAAGGAGAT | ATACATATGC | 300 |
| 301 | ATCATCACCA | TCACCATGGT | AAGCCTATCC | CTAACCCTCT | CCTCGGTCTC | 350 |
| 351 | GATTCTACGG | AAAACCTGTA | TTTTCAGGGA | ATTGATCCCT | TCACCAAGGG | 400 |
| 401 | CGAGCTCAGA | TCCGGCTGCT | AACAAAGCCC | GAAAGGAAGC | TGAGTTGGCT | 450 |
| 451 | GCTGCCACCG | CTGAGCAATA | ACTAGCATAA | CCCCTTGGGG | CCTCTAAACG | 500 |
| 501 | GGTCTTGAGG | GGTTTTTTGC | TGAAAGGAGG | AACTATATCC | GGATATCCCG | 550 |
| 551 | CAAGAGGCCC | GGCAGTACCG | GCATAACCAA | GCCTATGCCT | ACAGCATCCA | 600 |
| 601 | GGGTGACGGT | GCCGAGGATG | ACGATGAGCG | CATTGTTAGA | TTTCATACAC | 650 |
| 651 | GGTGCCTGAC | TGCGTTAGCA | ATTTAACTGT | GATAAACTAC | CGCATTAAAG | 700 |
| 701 | CTAGCTTATC | GATGATAAGC | TGTCAAACAT | GAGAATTAAT | TCTTGAAGAC | 750 |
| 751 | GAAAGGGCCT | CGTGATACGC | CTATTTTTAT | AGGTTAATGT | CATGATAATA | 800 |
| 801 | ATGGTTTCTT | AGACGTCAGG | TGGCACTTTT | CGGGGAAATG | TGCGCGGAAC | 850 |
| 851 | CCCTATTTGT | TTATTTTTCT | AAATACATTC | AAATATGTAT | CCGCTCATGA | 900 |
| 901 | GACAATAACC | CTGATAAATG | CTTCAATAAT | ATTGAAAAAG | GAAGAGTATG | 950 |
| 951 | AGTATTCAAC | ATTTCCGTGT | CGCCCTTATT | CCCTTTTTTG | CGGCATTTTG | 1000 |
| 1001 | CCTTCCTGTT | TTTGCTCACC | CAGAAACGCT | GGTGAAAGTA | AAAGATGCTG | 1050 |
| 1051 | AAGATCAGTT | GGGTGCACGA | GTGGGTTACA | TCGAACTGGA | TCTCAACAGC | 1100 |
| 1101 | GGTAAGATCC | TTGAGAGTTT | TCGCCCCGAA | GAACGTTTTC | CAATGATGAG | 1150 |
| 1151 | CACTTTTAAA | GTTCTGCTAT | GTGGCGCGGT | ATTATCCCGT | GTTGACGCCG | 1200 |
| 1201 | GGCAAGAGCA | ACTCGGTCGC | CGCATACACT | ATTCTCAGAA | TGACTTGGTT | 1250 |
| 1251 | GAGTACTCAC | CAGTCACAGA | AAAGCATCTT | ACGGATGGCA | TGACAGTAAG | 1300 |
| 1301 | AGAATTATGC | AGTGCTGCCA | TAACCATGAG | TGATAACACT | GCGGCCAACT | 1350 |
| 1351 | TACTTCTGAC | AACGATCGGA | GGACCGAAGG | AGCTAACCGC | TTTTTTGCAC | 1400 |
| 1401 | AACATGGGGG | ATCATGTAAC | TCGCCTTGAT | CGTTGGGAAC | CGGAGCTGAA | 1450 |
| 1451 | TGAAGCCATA | CCAAACGACG | AGCGTGACAC | CACGATGCCT | GCAGCAATGG | 1500 |
| 1501 | CAACAACGTT | GCGCAAACTA | TTAACTGGCG | AACTACTTAC | TCTAGCTTCC | 1550 |
| 1551 | CGGCAACAAT | TAATAGACTG | GATGGAGGCG | GATAAAGTTG | CAGGACCACT | 1600 |
| 1601 | TCTGCGCTCG | GCCCTTCCGG | CTGGCTGGTT | TATTGCTGAT | AAATCTGGAG | 1650 |
| 1651 | CCGGTGAGCG | TGGGTCTCGC | GGTATCATTG | CAGCACTGGG | GCCAGATGGT | 1700 |
| 1701 | AAGCCCTCCC | GTATCGTAGT | TATCTACACG | ACGGGGAGTC | AGGCAACTAT | 1750 |
| 1751 | GGATGAACGA | AATAGACAGA | TCGCTGAGAT | AGGTGCCTCA | CTGATTAAGC | 1800 |
| 1801 | ATTGGTAACT | GTCAGACCAA | GTTTACTCAT | ATATACTTTA | GATTGATTTA | 1850 |
| 1851 | AAACTTCATT | TTTAATTTAA | AAGGATCTAG | GTGAAGATCC | TTTTTGATAA | 1900 |
| 1901 | TCTCATGACC | AAAATCCCTT | AACGTGAGTT | TTCGTTCCAC | TGAGCGTCAG | 1950 |
| 1951 | ACCCCGTAGA | AAAGATCAAA | GGATCTTCTT | GAGATCCTTT | TTTTCTGCGC | 2000 |
| 2001 | GTAATCTGCT | GCTTGCAAAC | AAAAAAACCA | CCGCTACCAG | CGGTGGTTTG | 2050 |
| 2051 | TTTGCCGGAT | CAAGAGCTAC | CAACTCTTTT | TCCGAAGGTA | ACTGGCTTCA | 2100 |
| 2101 | GCAGAGCGCA | GATACCAAAT | ACTGTCCTTC | TAGTGTAGCC | GTAGTTAGGC | 2150 |
| 2151 | CACCACTTCA | AGAACTCTGT | AGCACCGCCT | ACATACCTCG | CTCTGCTAAT | 2200 |
| 2201 | CCTGTTACCA | GTGGCTGCTG | CCAGTGGCGA | TAAGTCGTGT | CTTACCGGGT | 2250 |
| 2251 | TGGACTCAAG | ACGATAGTTA | CCGGATAAGG | CGCAGCGGTC | GGGCTGAACG | 2300 |
| 2301 | GGGGGTTCGT | GCACACAGCC | CAGCTTGGAG | CGAACGACCT | ACACCGAACT | 2350 |
| 2351 | GAGATACCTA | CAGCGTGAGC | TATGAGAAAG | CGCCACGCTT | CCCGAAGGGA | 2400 |
| 2401 | GAAAGGCGGA | CAGGTATCCG | GTAAGCGGCA | GGGTCGGAAC | AGGAGAGCGC | 2450 |
| 2451 | ACGAGGGAGC | TTCCAGGGGG | AAACGCCTGG | TATCTTTATA | GTCCTGTCGG | 2500 |
| 2501 | GTTTCGCCAC | CTCTGACTTG | AGCGTCGATT | TTTGTGATGC | TCGTCAGGGG | 2550 |
| 2551 | GGCGGAGCCT | ATGGAAAAAC | GCCAGCAACG | CGGCCTTTTT | ACGGTTCCTG | 2600 |
| 2601 | GCCTTTTGCT | GGCCTTTTGC | TCACATGTTC | TTTCCTGCGT | TATCCCCTGA | 2650 |
| 2651 | TTCTGTGGAT | AACCGTATTA | CCGCCTTTGA | GTGAGCTGAT | ACCGCTCGCC | 2700 |
| 2701 | GCAGCCGAAC | GACCGAGCGC | AGCGAGTCAG | TGAGCGAGGA | AGCGGAAGAG | 2750 |
| 2751 | CGCCTGATGC | GGTATTTTCT | CCTTACGCAT | CTGTGCGGTA | TTTCACACCG | 2800 |
| 2801 | CATATATGGT | GCACTCTCAG | TACAATCTGC | TCTGATGCCG | CATAGTTAAG | 2850 |
| 2851 | CCAGTATACA | CTCCGCTATC | GCTACGTGAC | TGGGTCATGG | CTGCGCCCCG | 2900 |
| 2901 | ACACCCGCCA | ACACCCGCTG | ACGCGCCCTG | ACGGGCTTGT | CTGCTCCCGG | 2950 |
| 2951 | CATCCGCTTA | CAGACAAGCT | GTGACCGTCT | CCGGGAGCTG | CATGTGTCAG | 3000 |
| 3001 | AGGTTTTCAC | CGTCATCACC | GAAACGCGCG | AGGCAGCTGC | GGTAAAGCTC | 3050 |
| 3051 | ATCAGCGTGG | TCGTGAAGCG | ATTCACAGAT | GTCTGCCTGT | TCATCCGCGT | 3100 |
| 3101 | CCAGCTCGTT | GAGTTTCTCC | AGAAGCGTTA | ATGTCTGGCT | TCTGATAAAG | 3150 |
| 3151 | CGGGCCATGT | TAAGGGCGGT | TTTTTCCTGT | TTGGTCACTG | ATGCCTCCGT | 3200 |
| 3201 | GTAAGGGGGA | TTTCTGTTCA | TGGGGGTAAT | GATACCGATG | AAACGAGAGA | 3250 |
| 3251 | GGATGCTCAC | GATACGGGTT | ACTGATGATG | AACATGCCCG | GTTACTGGAA | 3300 |
| 3301 | CGTTGTGAGG | GTAAACAACT | GGCGGTATGG | ATGCGGCGGG | ACCAGAGAAA | 3350 |
| 3351 | AATCACTCAG | GGTCAATGCC | AGCGCTTCGT | TAATACAGAT | GTAGGTGTTC | 3400 |
| 3401 | CACAGGGTAG | CCAGCAGCAT | CCTGCGATGC | AGATCCGGAA | CATAATGGTG | 3450 |
| 3451 | CAGGGCGCTG | ACTTCCGCGT | TTCCAGACTT | TACGAAACAC | GGAAACCGAA | 3500 |
| 3501 | GACCATTCAT | GTTGTTGCTC | AGGTCGCAGA | CGTTTTGCAG | CAGCAGTCGC | 3550 |
| 3551 | TTCACGTTCG | CTCGCGTATC | GGTGATTCAT | TCTGCTAACC | AGTAAGGCAA | 3600 |
| 3601 | CCCCGCCAGC | CTAGCCGGGT | CCTCAACGAC | AGGAGCACGA | TCATGCGCAC | 3650 |
| 3651 | CCGTGGCCAG | GACCCAACGC | TGCCCGAGAT | GCGCCGCGTG | CGGCTGCTGG | 3700 |
| 3701 | AGATGGCGGA | CGCGATGGAT | ATGTTCTGCC | AAGGGTTGGT | TTGCGCATTC | 3750 |
| 3751 | ACAGTTCTCC | GCAAGAATTG | ATTGGCTCCA | ATTCTTGGAG | TGGTGAATCC | 3800 |
| 3801 | GTTAGCGAGG | TGCCGCCGGC | TTCCATTCAG | GTCGAGGTGG | CCCGGCTCCA | 3850 |
| 3851 | TGCACCGCGA | CGCAACGCGG | GGAGGCAGAC | AAGGTATAGG | GCGGCGCCTA | 3900 |
| 3901 | CAATCCATGC | CAACCCGTTC | CATGTGCTCG | CCGAGGCGGC | ATAAATCGCC | 3950 |
| 3951 | GTGACGATCA | GCGGTCCAGT | GATCGAAGTT | AGGCTGGTAA | GAGCCGCGAG | 4000 |
| 4001 | CGATCCTTGA | AGCTGTCCCT | GATGGTCGTC | ATCTACCTGC | CTGGACAGCA | 4050 |
| 4051 | TGGCCTGCAA | CGCGGGCATC | CCGATGCCGC | CGGAAGCGAG | AAGAATCATA | 4100 |
| 4101 | ATGGGGAAGG | CCATCCAGCC | TCGCGTCGCG | AACGCCAGCA | AGACGTAGCC | 4150 |
| 4151 | CAGCGCGTCG | GCCGCCATGC | CGGCGATAAT | GGCCTGCTTC | TCGCCGAAAC | 4200 |
| 4201 | GTTTGGTGGC | GGGACCAGTG | ACGAAGGCTT | GAGCGAGGGC | GTGCAAGATT | 4250 |
| 4251 | CCGAATACCG | CAAGCGACAG | GCCGATCATC | GTCGCGCTCC | AGCGAAAGCG | 4300 |
| 4301 | GTCCTCGCCG | AAAATGACCC | AGAGCGCTGC | CGGCACCTGT | CCTACGAGTT | 4350 |
| 4351 | GCATGATAAA | GAAGACAGTC | ATAAGTGCGG | CGACGATAGT | CATGCCCCGC | 4400 |
| 4401 | GCCCACCGGA | AGGAGCTGAC | TGGGTTGAAG | GCTCTCAAGG | GCATCGGTCG | 4450 |
| 4451 | AGATCCCGGT | GCCTAATGAG | TGAGCTAACT | TACATTAATT | GCGTTGCGCT | 4500 |
| 4501 | CACTGCCCGC | TTTCCAGTCG | GGAAACCTGT | CGTGCCAGCT | GCATTAATGA | 4550 |
| 4551 | ATCGGCCAAC | GCGCGGGGAG | AGGCGGTTTG | CGTATTGGGC | GCCAGGGTGG | 4600 |
| 4601 | TTTTTCTTTT | CACCAGTGAG | ACGGGCAACA | GCTGATTGCC | CTTCACCGCC | 4650 |
| 4651 | TGGCCCTGAG | AGAGTTGCAG | CAAGCGGTCC | ACGCTGGTTT | GCCCCAGCAG | 4700 |
| 4701 | GCGAAAATCC | TGTTTGATGG | TGGTTAACGG | CGGGATATAA | CATGAGCTGT | 4750 |
| 4751 | CTTCGGTATC | GTCGTATCCC | ACTACCGAGA | TATCCGCACC | AACGCGCAGC | 4800 |
| 4801 | CCGGACTCGG | TAATGGCGCG | CATTGCGCCC | AGCGCCATCT | GATCGTTGGC | 4850 |
| 4851 | AACCAGCATC | GCAGTGGGAA | CGATGCCCTC | ATTCAGCATT | TGCATGGTTT | 4900 |
| 4901 | GTTGAAAACC | GGACATGGCA | CTCCAGTCGC | CTTCCCGTTC | CGCTATCGGC | 4950 |
| 4951 | TGAATTTGAT | TGCGAGTGAG | ATATTTATGC | CAGCCAGCCA | GACGCAGACG | 5000 |
| 5001 | CGCCGAGACA | GAACTTAATG | GGCCCGCTAA | CAGCGCGATT | TGCTGGTGAC | 5050 |
| 5051 | CCAATGCGAC | CAGATGCTCC | ACGCCCAGTC | GCGTACCGTC | TTCATGGGAG | 5100 |
| 5101 | AAAATAATAC | TGTTGATGGG | TGTCTGGTCA | GAGACATCAA | GAAATAACGC | 5150 |
| 5151 | CGGAACATTA | GTGCAGGCAG | CTTCCACAGC | AATGGCATCC | TGGTCATCCA | 5200 |
| 5201 | GCGGATAGTT | AATGATCAGC | CCACTGACGC | GTTGCGCGAG | AAGATTGTGC | 5250 |
| 5251 | ACCGCCGCTT | TACAGGCTTC | GACGCCGCTT | CGTTCTACCA | TCGACACCAC | 5300 |
| 5301 | CACGCTGGCA | CCCAGTTGAT | CGGCGCGAGA | TTTAATCGCC | GCGACAATTT | 5350 |
| 5351 | GCGACGGCGC | GTGCAGGGCC | AGACTGGAGG | TGGCAACGCC | AATCAGCAAC | 5400 |
| 5401 | GACTGTTTGC | CCGCCAGTTG | TTGTGCCACG | CGGTTGGGAA | TGTAATTCAG | 5450 |
| 5451 | CTCCGCCATC | GCCGCTTCCA | CTTTTTCCCG | CGTTTTCGCA | GAAACGTGGC | 5500 |
| 5501 | TGGCCTGGTT | CACCACGCGG | GAAACGGTCT | GATAAGAGAC | ACCGGCATAC | 5550 |
| 5551 | TCTGCGACAT | CGTATAACGT | TACTGGTTTC | ACATTCACCA | CCCTGAATTG | 5600 |
| 5601 | ACTCTCTTCC | GGGCGCTATC | ATGCCATACC | GCGAAAGGTT | TTGCGCCATT | 5650 |
| 5651 | CGATGGTGTC | CGGGATCTCG | ACGCTCTCCC | TTATGCGACT | CCTGCATTAG | 5700 |
| 5701 | GAAGCAGCCC | AGTAGTAGGT | TGAGGCCGTT | GAGCACCGCC | GCCGCAAGGA | 5750 |
| 5751 | ATGGTGCATG | 5760 |
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文献和实验中,现在Invitrogen已经克服了这个问题。在载体上多加四个碱基GTGG,就是这四个碱基使它可以与PCR产物定向连接。其作用机理见下图。 结合TOPO技术的有五个系列载体:pET100/D-TOPO®、pET101/D-TOPO®、pET102/D-TOPO®、pET151/D-TOPO®和pET200/D-TOPO®,这五个载体根据所带的标记、是否具有蛋白酶切位点而各有不同。详细信息参见下图。 结合TOPO技术的有五个系列载体:pET100/D
or Directional TOPO® Cloning, optimal results are achieved most often when using a 1:10 dilution of the PCR product.Fresh PCR ProductThe use of fresh PCR products in TA, TOPO® TA, and Directional TOPO® Cloning is recommended due the potential presence
Gateway兼容cDNA文库 (4)Gateway改造过的克隆资源* * 这些克隆资源和cDNA文库两边加有attB位点。这些克隆可以通过与供载体及BP Gateway酶反应转换到入门载体。获得已有克隆资源的更多信息。 三、PCR定向(PCR-Directional)TOPO克隆 定向TOPO克隆使得克隆PCR产物和其它的DNA分子更加快速和有效。进行5分钟的简单连接,产生>90%的重组子。您不仅会比用连接酶介导的方法更快的获得克隆,而且可以节省因第一次无结果而重复试验所额外浪费的时间。 定向
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