pET-48b(+)

名称	编号	规格	价格	手册
pET-48b(+)(pET48b)（60908-2815）y	60908-2815y

质粒编号: 60908-2815y
质粒名称: pET-48b(+)
其他名称: pET48b, pET 48b
质粒大小（bp）: 5605
抗性: Kanamycin
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: 高
蛋白标签: N-Trx, N-His,N-HRV 3C, C-S, C-Thrombin
5′ 测序引物: T7或者Trx-F

5′ 测序引物序列: T7: 5′-TAATACGACTCACTATAGGG-3′; Trx-F: 5′-TTCCTCGACGCTAACCTG-3′

3′ 测序引物: T7t

3′ 测序引物序列: T7t: 5′-TGCTAGTTATTGCTCAGCGG-3′
GenBank号:
其他属性: Same as pET47 but also has Nterm Trx Tag; contains HRV 3C Protease cleavage site for fusion tag removal at low temperatures; Cterm thrombin cleavage site.
质粒图谱:

Feature Name	Start	End
T7_terminator	120	1
T7_Terminal_primer	69	87
S15	212	168
6xHIS	359	342
T7_transl_en_RBS	792	776
lacO	837	810
T7_promoter	855	837
tet (300 – 563)	891	1154
pBRrevBam_primer	962	943
lacI	1237	2328
ROP	2900	3091
pGEX_3_primer	3107	3085
pBR322_origin	4125	3506
KanR2	4231	5046
f1_origin	5577	5271

ORF	Start	End
ORF frame 3	767	141
ORF frame 3	345	1496
ORF frame 1	1369	2328
ORF frame 1	4231	5046

Enzyme Name	Cut
PacI	141
XhoI	147
BstBI	187
SacI	238
NotI	242
EagI	242
HindIII	249
SalI	255
AatII	266
AscI	270
StuI	280
EcoRI	289
BamHI	295
KpnI	306
XmaI	306
SmaI	308
SacII	338
SpeI	417
MscI	426
NdeI	766
XbaI	804
BclI	1610
ApaI	1807
HpaI	2102
NruI	4319

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgtttagcag	cctaggttaa	ttaagcctcg	150
151	agagcagcag	aagtagagct	gtccatgtgc	tggcgttcga	atttagcagc	200
201	agcggtttct	ttactaccgc	gtggcaccag	agcgagctct	gcggccgcaa	250
251	gcttgtcgac	ggacgtcggg	cgcgccaagg	cctgtacaga	attcggatcc	300
301	tggtacccgg	gtccctgaaa	gaggacttca	agagccgcgg	agtgatggtg	350
351	atggtgatga	ccagaaccac	tagatggagt	aggagtagga	ggattgttat	400
401	tagaaccgcc	accaccacta	gtatggccag	aaccagaacc	ggccaggtta	450
451	gcgtcgagga	actctttcaa	ctgaccttta	gacagtgcac	ccactttggt	500
501	tgccgccact	tcaccgtttt	tgaacagcag	cagagtcggg	ataccacgga	550
551	tgccatattt	cggcgcagtg	ccagggtttt	gatcgatgtt	cagttttgca	600
601	acggtcagtt	tgccctgata	ttcgtcagcg	atttcatcca	gaatcggggc	650
651	gatcattttg	cacggaccgc	accactctgc	ccagaaatcg	acgaggatcg	700
701	ccccgtccgc	tttgagtaca	tccgtgtcaa	aactgtcgtc	agtcaggtga	750
751	ataattttat	cgctcatatg	tatatctcct	tcttaaagtt	aaacaaaatt	800
801	atttctagag	gggaattgtt	atccgctcac	aattccccta	tagtgagtcg	850
851	tattaatttc	gcgggatcga	gatcgatctc	gatcctctac	gccggacgca	900
901	tcgtggccgg	catcaccggc	gccacaggtg	cggttgctgg	cgcctatatc	950
951	gccgacatca	ccgatgggga	agatcgggct	cgccacttcg	ggctcatgag	1000
1001	cgcttgtttc	ggcgtgggta	tggtggcagg	ccccgtggcc	gggggactgt	1050
1051	tgggcgccat	ctccttgcat	gcaccattcc	ttgcggcggc	ggtgctcaac	1100
1101	ggcctcaacc	tactactggg	ctgcttccta	atgcaggagt	cgcataaggg	1150
1151	agagcgtcga	gatcccggac	accatcgaat	ggcgcaaaac	ctttcgcggt	1200
1201	atggcatgat	agcgcccgga	agagagtcaa	ttcagggtgg	tgaatgtgaa	1250
1251	accagtaacg	ttatacgatg	tcgcagagta	tgccggtgtc	tcttatcaga	1300
1301	ccgtttcccg	cgtggtgaac	caggccagcc	acgtttctgc	gaaaacgcgg	1350
1351	gaaaaagtgg	aagcggcgat	ggcggagctg	aattacattc	ccaaccgcgt	1400
1401	ggcacaacaa	ctggcgggca	aacagtcgtt	gctgattggc	gttgccacct	1450
1451	ccagtctggc	cctgcacgcg	ccgtcgcaaa	ttgtcgcggc	gattaaatct	1500
1501	cgcgccgatc	aactgggtgc	cagcgtggtg	gtgtcgatgg	tagaacgaag	1550
1551	cggcgtcgaa	gcctgtaaag	cggcggtgca	caatcttctc	gcgcaacgcg	1600
1601	tcagtgggct	gatcattaac	tatccgctgg	atgaccagga	tgccattgct	1650
1651	gtggaagctg	cctgcactaa	tgttccggcg	ttatttcttg	atgtctctga	1700
1701	ccagacaccc	atcaacagta	ttattttctc	ccatgaagac	ggtacgcgac	1750
1751	tgggcgtgga	gcatctggtc	gcattgggtc	accagcaaat	cgcgctgtta	1800
1801	gcgggcccat	taagttctgt	ctcggcgcgt	ctgcgtctgg	ctggctggca	1850
1851	taaatatctc	actcgcaatc	aaattcagcc	gatagcggaa	cgggaaggcg	1900
1901	actggagtgc	catgtccggt	tttcaacaaa	ccatgcaaat	gctgaatgag	1950
1951	ggcatcgttc	ccactgcgat	gctggttgcc	aacgatcaga	tggcgctggg	2000
2001	cgcaatgcgc	gccattaccg	agtccgggct	gcgcgttggt	gcggacatct	2050
2051	cggtagtggg	atacgacgat	accgaagaca	gctcatgtta	tatcccgccg	2100
2101	ttaaccacca	tcaaacagga	ttttcgcctg	ctggggcaaa	ccagcgtgga	2150
2151	ccgcttgctg	caactctctc	agggccaggc	ggtgaagggc	aatcagctgt	2200
2201	tgcccgtctc	actggtgaaa	agaaaaacca	ccctggcgcc	caatacgcaa	2250
2251	accgcctctc	cccgcgcgtt	ggccgattca	ttaatgcagc	tggcacgaca	2300
2301	ggtttcccga	ctggaaagcg	ggcagtgagc	gcaacgcaat	taatgtaagt	2350
2351	tagctcactc	attaggcacc	gggatctcga	ccgatgccct	tgagagcctt	2400
2401	caacccagtc	agctccttcc	ggtgggcgcg	gggcatgact	agcatgatcg	2450
2451	tgctcctgtc	gttgaggacc	cggctaggct	ggcggggttg	ccttactggt	2500
2501	tagcagaatg	aatcaccgat	acgcgagcga	acgtgaagcg	actgctgctg	2550
2551	caaaacgtct	gcgacctgag	caacaacatg	aatggtcttc	ggtttccgtg	2600
2601	tttcgtaaag	tctggaaacg	cggaagtcag	cgccctgcac	cattatgttc	2650
2651	cggatctgca	tcgcaggatg	ctgctggcta	ccctgtggaa	cacctacatc	2700
2701	tgtattaacg	aagcgctggc	attgaccctg	agtgattttt	ctctggtccc	2750
2751	gccgcatcca	taccgccagt	tgtttaccct	cacaacgttc	cagtaaccgg	2800
2801	gcatgttcat	catcagtaac	ccgtatcgtg	agcatcctct	ctcgtttcat	2850
2851	cggtatcatt	acccccatga	acagaaatcc	cccttacacg	gaggcatcag	2900
2901	tgaccaaaca	ggaaaaaacc	gcccttaaca	tggcccgctt	tatcagaagc	2950
2951	cagacattaa	cgcttctgga	gaaactcaac	gagctggacg	cggatgaaca	3000
3001	ggcagacatc	tgtgaatcgc	ttcacgacca	cgctgatgag	ctttaccgca	3050
3051	gctgcctcgc	gcgtttcggt	gatgacggtg	aaaacctctg	acacatgcag	3100
3101	ctcccggaga	cggtcacagc	ttgtctgtaa	gcggatgccg	ggagcagaca	3150
3151	agcccgtcag	ggcgcgtcag	cgggtgttgg	cgggtgtcgg	ggcgcagcca	3200
3201	tgacccagtc	acgtagcgat	agcggagtgt	atactggctt	aactatgcgg	3250
3251	catcagagca	gattgtactg	agagtgcacc	atatatgcgg	tgtgaaatac	3300
3301	cgcacagatg	cgtaaggaga	aaataccgca	tcaggcgctc	ttccgcttcc	3350
3351	tcgctcactg	actcgctgcg	ctcggtcgtt	cggctgcggc	gagcggtatc	3400
3401	agctcactca	aaggcggtaa	tacggttatc	cacagaatca	ggggataacg	3450
3451	caggaaagaa	catgtgagca	aaaggccagc	aaaaggccag	gaaccgtaaa	3500
3501	aaggccgcgt	tgctggcgtt	tttccatagg	ctccgccccc	ctgacgagca	3550
3551	tcacaaaaat	cgacgctcaa	gtcagaggtg	gcgaaacccg	acaggactat	3600
3601	aaagatacca	ggcgtttccc	cctggaagct	ccctcgtgcg	ctctcctgtt	3650
3651	ccgaccctgc	cgcttaccgg	atacctgtcc	gcctttctcc	cttcgggaag	3700
3701	cgtggcgctt	tctcatagct	cacgctgtag	gtatctcagt	tcggtgtagg	3750
3751	tcgttcgctc	caagctgggc	tgtgtgcacg	aaccccccgt	tcagcccgac	3800
3801	cgctgcgcct	tatccggtaa	ctatcgtctt	gagtccaacc	cggtaagaca	3850
3851	cgacttatcg	ccactggcag	cagccactgg	taacaggatt	agcagagcga	3900
3901	ggtatgtagg	cggtgctaca	gagttcttga	agtggtggcc	taactacggc	3950
3951	tacactagaa	ggacagtatt	tggtatctgc	gctctgctga	agccagttac	4000
4001	cttcggaaaa	agagttggta	gctcttgatc	cggcaaacaa	accaccgctg	4050
4051	gtagcggtgg	tttttttgtt	tgcaagcagc	agattacgcg	cagaaaaaaa	4100
4101	ggatctcaag	aagatccttt	gatcttttct	acggggtctg	acgctcagtg	4150
4151	gaacgaaaac	tcacgttaag	ggattttggt	catgaacaat	aaaactgtct	4200
4201	gcttacataa	acagtaatac	aaggggtgtt	atgagccata	ttcaacggga	4250
4251	aacgtcttgc	tctaggccgc	gattaaattc	caacatggat	gctgatttat	4300
4301	atgggtataa	atgggctcgc	gataatgtcg	ggcaatcagg	tgcgacaatc	4350
4351	tatcgattgt	atgggaagcc	cgatgcgcca	gagttgtttc	tgaaacatgg	4400
4401	caaaggtagc	gttgccaatg	atgttacaga	tgagatggtc	agactaaact	4450
4451	ggctgacgga	atttatgcct	cttccgacca	tcaagcattt	tatccgtact	4500
4501	cctgatgatg	catggttact	caccactgcg	atccccggca	aaacagcatt	4550
4551	ccaggtatta	gaagaatatc	ctgattcagg	tgaaaatatt	gttgatgcgc	4600
4601	tggcagtgtt	cctgcgccgg	ttgcattcga	ttcctgtttg	taattgtcct	4650
4651	tttaacagtg	atcgcgtatt	tcgtctcgct	caggcgcaat	cacgaatgaa	4700
4701	taacggtttg	gttgatgcga	gtgattttga	tgacgagcgt	aatggctggc	4750
4751	ctgttgaaca	agtctggaaa	gaaatgcata	aacttttgcc	attctcaccg	4800
4801	gattcagtcg	tcactcatgg	tgatttctca	cttgataacc	ttatttttga	4850
4851	cgaggggaaa	ttaataggtt	gtattgatgt	tggacgagtc	ggaatcgcag	4900
4901	accgatacca	ggatcttgcc	atcctatgga	actgcctcgg	tgagttttct	4950
4951	ccttcattac	agaaacggct	ttttcaaaaa	tatggtattg	ataatcctga	5000
5001	tatgaataaa	ttgcagtttc	atttgatgct	cgatgagttt	ttctaagaat	5050
5051	taattcatga	gcggatacat	atttgaatgt	atttagaaaa	ataaacaaat	5100
5101	aggggttccg	cgcacatttc	cccgaaaagt	gccacctgaa	attgtaaacg	5150
5151	ttaatatttt	gttaaaattc	gcgttaaatt	tttgttaaat	cagctcattt	5200
5201	tttaaccaat	aggccgaaat	cggcaaaatc	ccttataaat	caaaagaata	5250
5251	gaccgagata	gggttgagtg	ttgttccagt	ttggaacaag	agtccactat	5300
5301	taaagaacgt	ggactccaac	gtcaaagggc	gaaaaaccgt	ctatcagggc	5350
5351	gatggcccac	tacgtgaacc	atcaccctaa	tcaagttttt	tggggtcgag	5400
5401	gtgccgtaaa	gcactaaatc	ggaaccctaa	agggagcccc	cgatttagag	5450
5451	cttgacgggg	aaagccggcg	aacgtggcga	gaaaggaagg	gaagaaagcg	5500
5501	aaaggagcgg	gcgctagggc	gctggcaagt	gtagcggtca	cgctgcgcgt	5550
5551	aaccaccaca	cccgccgcgc	ttaatgcgcc	gctacagggc	gcgtcccatt	5600
5601	cgcca	5605