pET-29c(+)

名称	编号	规格	价格	手册
pET-29c(+)(pET29c)

质粒编号:
质粒名称: pET-29c(+)
其他名称:
质粒大小（bp）: 5372
抗性: Tet;Kan
质粒类型: 大肠杆菌表达载体
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平: 高
蛋白标签: N-S, N-Thrombin, C-His
5′ 测序引物:T7

5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性:

质粒图谱:

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgttagcagc	cggatctcag	tggtggtggt	150
151	ggtggtgctc	gagtgcggcc	gcaagcttgt	cgacggagct	cgaattcgga	200
201	tccacagata	tcccatggaa	ccgcgtggca	ccagggtacc	cagatctggg	250
251	ctgtccatgt	gctggcgttc	gaatttagca	gcagcggttt	ctttcatatg	300
301	tatatctcct	tcttaaagtt	aaacaaaatt	atttctagag	gggaattgtt	350
351	atccgctcac	aattccccta	tagtgagtcg	tattaatttc	gcgggatcga	400
401	gatcgatctc	gatcctctac	gccggacgca	tcgtggccgg	catcaccggc	450
451	gccacaggtg	cggttgctgg	cgcctatatc	gccgacatca	ccgatgggga	500
501	agatcgggct	cgccacttcg	ggctcatgag	cgcttgtttc	ggcgtgggta	550
551	tggtggcagg	ccccgtggcc	gggggactgt	tgggcgccat	ctccttgcat	600
601	gcaccattcc	ttgcggcggc	ggtgctcaac	ggcctcaacc	tactactggg	650
651	ctgcttccta	atgcaggagt	cgcataaggg	agagcgtcga	gatcccggac	700
701	accatcgaat	ggcgcaaaac	ctttcgcggt	atggcatgat	agcgcccgga	750
751	agagagtcaa	ttcagggtgg	tgaatgtgaa	accagtaacg	ttatacgatg	800
801	tcgcagagta	tgccggtgtc	tcttatcaga	ccgtttcccg	cgtggtgaac	850
851	caggccagcc	acgtttctgc	gaaaacgcgg	gaaaaagtgg	aagcggcgat	900
901	ggcggagctg	aattacattc	ccaaccgcgt	ggcacaacaa	ctggcgggca	950
951	aacagtcgtt	gctgattggc	gttgccacct	ccagtctggc	cctgcacgcg	1000
1001	ccgtcgcaaa	ttgtcgcggc	gattaaatct	cgcgccgatc	aactgggtgc	1050
1051	cagcgtggtg	gtgtcgatgg	tagaacgaag	cggcgtcgaa	gcctgtaaag	1100
1101	cggcggtgca	caatcttctc	gcgcaacgcg	tcagtgggct	gatcattaac	1150
1151	tatccgctgg	atgaccagga	tgccattgct	gtggaagctg	cctgcactaa	1200
1201	tgttccggcg	ttatttcttg	atgtctctga	ccagacaccc	atcaacagta	1250
1251	ttattttctc	ccatgaagac	ggtacgcgac	tgggcgtgga	gcatctggtc	1300
1301	gcattgggtc	accagcaaat	cgcgctgtta	gcgggcccat	taagttctgt	1350
1351	ctcggcgcgt	ctgcgtctgg	ctggctggca	taaatatctc	actcgcaatc	1400
1401	aaattcagcc	gatagcggaa	cgggaaggcg	actggagtgc	catgtccggt	1450
1451	tttcaacaaa	ccatgcaaat	gctgaatgag	ggcatcgttc	ccactgcgat	1500
1501	gctggttgcc	aacgatcaga	tggcgctggg	cgcaatgcgc	gccattaccg	1550
1551	agtccgggct	gcgcgttggt	gcggacatct	cggtagtggg	atacgacgat	1600
1601	accgaagaca	gctcatgtta	tatcccgccg	ttaaccacca	tcaaacagga	1650
1651	ttttcgcctg	ctggggcaaa	ccagcgtgga	ccgcttgctg	caactctctc	1700
1701	agggccaggc	ggtgaagggc	aatcagctgt	tgcccgtctc	actggtgaaa	1750
1751	agaaaaacca	ccctggcgcc	caatacgcaa	accgcctctc	cccgcgcgtt	1800
1801	ggccgattca	ttaatgcagc	tggcacgaca	ggtttcccga	ctggaaagcg	1850
1851	ggcagtgagc	gcaacgcaat	taatgtaagt	tagctcactc	attaggcacc	1900
1901	gggatctcga	ccgatgccct	tgagagcctt	caacccagtc	agctccttcc	1950
1951	ggtgggcgcg	gggcatgact	atcgtcgccg	cacttatgac	tgtcttcttt	2000
2001	atcatgcaac	tcgtaggaca	ggtgccggca	gcgctctggg	tcattttcgg	2050
2051	cgaggaccgc	tttcgctgga	gcgcgacgat	gatcggcctg	tcgcttgcgg	2100
2101	tattcggaat	cttgcacgcc	ctcgctcaag	ccttcgtcac	tggtcccgcc	2150
2151	accaaacgtt	tcggcgagaa	gcaggccatt	atcgccggca	tggcggcccc	2200
2201	acgggtgcgc	atgatcgtgc	tcctgtcgtt	gaggacccgg	ctaggctggc	2250
2251	ggggttgcct	tactggttag	cagaatgaat	caccgatacg	cgagcgaacg	2300
2301	tgaagcgact	gctgctgcaa	aacgtctgcg	acctgagcaa	caacatgaat	2350
2351	ggtcttcggt	ttccgtgttt	cgtaaagtct	ggaaacgcgg	aagtcagcgc	2400
2401	cctgcaccat	tatgttccgg	atctgcatcg	caggatgctg	ctggctaccc	2450
2451	tgtggaacac	ctacatctgt	attaacgaag	cgctggcatt	gaccctgagt	2500
2501	gatttttctc	tggtcccgcc	gcatccatac	cgccagttgt	ttaccctcac	2550
2551	aacgttccag	taaccgggca	tgttcatcat	cagtaacccg	tatcgtgagc	2600
2601	atcctctctc	gtttcatcgg	tatcattacc	cccatgaaca	gaaatccccc	2650
2651	ttacacggag	gcatcagtga	ccaaacagga	aaaaaccgcc	cttaacatgg	2700
2701	cccgctttat	cagaagccag	acattaacgc	ttctggagaa	actcaacgag	2750
2751	ctggacgcgg	atgaacaggc	agacatctgt	gaatcgcttc	acgaccacgc	2800
2801	tgatgagctt	taccgcagct	gcctcgcgcg	tttcggtgat	gacggtgaaa	2850
2851	acctctgaca	catgcagctc	ccggagacgg	tcacagcttg	tctgtaagcg	2900
2901	gatgccggga	gcagacaagc	ccgtcagggc	gcgtcagcgg	gtgttggcgg	2950
2951	gtgtcggggc	gcagccatga	cccagtcacg	tagcgatagc	ggagtgtata	3000
3001	ctggcttaac	tatgcggcat	cagagcagat	tgtactgaga	gtgcaccata	3050
3051	tatgcggtgt	gaaataccgc	acagatgcgt	aaggagaaaa	taccgcatca	3100
3101	ggcgctcttc	cgcttcctcg	ctcactgact	cgctgcgctc	ggtcgttcgg	3150
3151	ctgcggcgag	cggtatcagc	tcactcaaag	gcggtaatac	ggttatccac	3200
3201	agaatcaggg	gataacgcag	gaaagaacat	gtgagcaaaa	ggccagcaaa	3250
3251	aggccaggaa	ccgtaaaaag	gccgcgttgc	tggcgttttt	ccataggctc	3300
3301	cgcccccctg	acgagcatca	caaaaatcga	cgctcaagtc	agaggtggcg	3350
3351	aaacccgaca	ggactataaa	gataccaggc	gtttccccct	ggaagctccc	3400
3401	tcgtgcgctc	tcctgttccg	accctgccgc	ttaccggata	cctgtccgcc	3450
3451	tttctccctt	cgggaagcgt	ggcgctttct	catagctcac	gctgtaggta	3500
3501	tctcagttcg	gtgtaggtcg	start= ttcgctccaa	gctgggctgt	gtgcacgaac	3550
3551	cccccgttca	gcccgaccgc	tgcgccttat	ccggtaacta	tcgtcttgag	3600
3601	tccaacccgg	taagacacga	cttatcgcca	ctggcagcag	ccactggtaa	3650
3651	caggattagc	agagcgaggt	atgtaggcgg	tgctacagag	ttcttgaagt	3700
3701	ggtggcctaa	ctacggctac	actagaagga	cagtatttgg	tatctgcgct	3750
3751	ctgctgaagc	cagttacctt	cggaaaaaga	gttggtagct	cttgatccgg	3800
3801	caaacaaacc	accgctggta	gcggtggttt	ttttgtttgc	aagcagcaga	3850
3851	ttacgcgcag	aaaaaaagga	tctcaagaag	atcctttgat	cttttctacg	3900
3901	gggtctgacg	ctcagtggaa	cgaaaactca	cgttaaggga	ttttggtcat	3950
3951	gaacaataaa	actgtctgct	tacataaaca	gtaatacaag	gggtgttatg	4000
4001	agccatattc	aacgggaaac	gtcttgctct	aggccgcgat	taaattccaa	4050
4051	catggatgct	gatttatatg	ggtataaatg	ggctcgcgat	aatgtcgggc	4100
4101	aatcaggtgc	gacaatctat	cgattgtatg	ggaagcccga	tgcgccagag	4150
4151	ttgtttctga	aacatggcaa	aggtagcgtt	gccaatgatg	ttacagatga	4200
4201	gatggtcaga	ctaaactggc	tgacggaatt	tatgcctctt	ccgaccatca	4250
4251	agcattttat	ccgtactcct	gatgatgcat	ggttactcac	cactgcgatc	4300
4301	cccgggaaaa	cagcattcca	ggtattagaa	gaatatcctg	attcaggtga	4350
4351	aaatattgtt	gatgcgctgg	cagtgttcct	gcgccggttg	cattcgattc	4400
4401	ctgtttgtaa	ttgtcctttt	aacagcgatc	gcgtatttcg	tctcgctcag	4450
4451	gcgcaatcac	gaatgaataa	cggtttggtt	gatgcgagtg	attttgatga	4500
4501	cgagcgtaat	ggctggcctg	ttgaacaagt	ctggaaagaa	atgcataaac	4550
4551	ttttgccatt	ctcaccggat	tcagtcgtca	ctcatggtga	tttctcactt	4600
4601	gataacctta	tttttgacga	ggggaaatta	ataggttgta	ttgatgttgg	4650
4651	acgagtcgga	atcgcagacc	gataccagga	tcttgccatc	ctatggaact	4700
4701	gcctcggtga	gttttctcct	tcattacaga	aacggctttt	tcaaaaatat	4750
4751	ggtattgata	atcctgatat	gaataaattg	cagtttcatt	tgatgctcga	4800
4801	tgagtttttc	taagaattaa	ttcatgagcg	gatacatatt	tgaatgtatt	4850
4851	tagaaaaata	aacaaatagg	ggttccgcgc	acatttcccc	gaaaagtgcc	4900
4901	acctgaaatt	gtaaacgtta	atattttgtt	aaaattcgcg	ttaaattttt	4950
4951	gttaaatcag	ctcatttttt	aaccaatagg	ccgaaatcgg	caaaatccct	5000
5001	tataaatcaa	aagaatagac	cgagataggg	ttgagtgttg	ttccagtttg	5050
5051	gaacaagagt	ccactattaa	agaacgtgga	ctccaacgtc	aaagggcgaa	5100
5101	aaaccgtcta	tcagggcgat	ggcccactac	gtgaaccatc	accctaatca	5150
5151	agttttttgg	ggtcgaggtg	ccgtaaagca	ctaaatcgga	accctaaagg	5200
5201	gagcccccga	tttagagctt	gacggggaaa	gccggcgaac	gtggcgagaa	5250
5251	aggaagggaa	gaaagcgaaa	ggagcgggcg	ctagggcgct	ggcaagtgta	5300
5301	gcggtcacgc	tgcgcgtaac	caccacaccc	gccgcgctta	atgcgccgct	5350
5351	acagggcgcg	tcccattcgc	ca	5372