pET-29b(+)

名称	编号	规格	价格	手册
pET-29b(+)(pET29b)	60908-3426y

质粒编号:60908-3426
质粒名称: pET-29b(+)
其他名称:
质粒大小（bp）: 5370
抗性: Kanamycin (卡那霉素)
质粒类型:大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-S, N-Thrombin, C-His
5′ 测序引物:T7

5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Nterm thrombin cleavage site; a,b,c vary by MCS.
质粒图谱:

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
lacI	217	1856
T7_transl_en_RBS	320	304
lacO	365	338
T7_promoter	383	365
tet (300 – 563)	419	682
pBRrevBam_primer	490	471
tet (576 – 851)	1915	2190
ROP	2665	2856
pGEX_3_primer	2872	2850
pBR322_origin	3890	3271
KanR2	3996	4811

ORF	Start	End
ORF frame 2	296	1024
ORF frame 3	897	1856
ORF frame 3	3996	4811

Enzyme Name	Cut
XhoI	158
NotI	166
EagI	166
HindIII	173
SalI	179
SacI	190
EcoRI	192
BamHI	198
EcoRV	206
NcoI	211
KpnI	237
BglII	240
BstBI	267
NdeI	294
XbaI	332
BclI	1138
ApaI	1335
HpaI	1630
FspI	2206
NruI	4084
SmaI	4301
XmaI	4299

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgttagcagc	cggatctcag	tggtggtggt	150
151	ggtggtgctc	gagtgcggcc	gcaagcttgt	cgacggagct	cgaattcgga	200
201	tccgatatcg	ccatggaacc	gcgtggcacc	agggtaccca	gatctgggct	250
251	gtccatgtgc	tggcgttcga	atttagcagc	agcggtttct	ttcatatgta	300
301	tatctccttc	ttaaagttaa	acaaaattat	ttctagaggg	gaattgttat	350
351	ccgctcacaa	ttcccctata	gtgagtcgta	ttaatttcgc	gggatcgaga	400
401	tcgatctcga	tcctctacgc	cggacgcatc	gtggccggca	tcaccggcgc	450
451	cacaggtgcg	gttgctggcg	cctatatcgc	cgacatcacc	gatggggaag	500
501	atcgggctcg	ccacttcggg	ctcatgagcg	cttgtttcgg	cgtgggtatg	550
551	gtggcaggcc	ccgtggccgg	gggactgttg	ggcgccatct	ccttgcatgc	600
601	accattcctt	gcggcggcgg	tgctcaacgg	cctcaaccta	ctactgggct	650
651	gcttcctaat	gcaggagtcg	cataagggag	agcgtcgaga	tcccggacac	700
701	catcgaatgg	cgcaaaacct	ttcgcggtat	ggcatgatag	cgcccggaag	750
751	agagtcaatt	cagggtggtg	aatgtgaaac	cagtaacgtt	atacgatgtc	800
801	gcagagtatg	ccggtgtctc	ttatcagacc	gtttcccgcg	tggtgaacca	850
851	ggccagccac	gtttctgcga	aaacgcggga	aaaagtggaa	gcggcgatgg	900
901	cggagctgaa	ttacattccc	aaccgcgtgg	cacaacaact	ggcgggcaaa	950
951	cagtcgttgc	tgattggcgt	tgccacctcc	agtctggccc	tgcacgcgcc	1000
1001	gtcgcaaatt	gtcgcggcga	ttaaatctcg	cgccgatcaa	ctgggtgcca	1050
1051	gcgtggtggt	gtcgatggta	gaacgaagcg	gcgtcgaagc	ctgtaaagcg	1100
1101	gcggtgcaca	atcttctcgc	gcaacgcgtc	agtgggctga	tcattaacta	1150
1151	tccgctggat	gaccaggatg	ccattgctgt	ggaagctgcc	tgcactaatg	1200
1201	ttccggcgtt	atttcttgat	gtctctgacc	agacacccat	caacagtatt	1250
1251	attttctccc	atgaagacgg	tacgcgactg	ggcgtggagc	atctggtcgc	1300
1301	attgggtcac	cagcaaatcg	cgctgttagc	gggcccatta	agttctgtct	1350
1351	cggcgcgtct	gcgtctggct	ggctggcata	aatatctcac	tcgcaatcaa	1400
1401	attcagccga	tagcggaacg	ggaaggcgac	tggagtgcca	tgtccggttt	1450
1451	tcaacaaacc	atgcaaatgc	tgaatgaggg	catcgttccc	actgcgatgc	1500
1501	tggttgccaa	cgatcagatg	gcgctgggcg	caatgcgcgc	cattaccgag	1550
1551	tccgggctgc	gcgttggtgc	ggacatctcg	gtagtgggat	acgacgatac	1600
1601	cgaagacagc	tcatgttata	tcccgccgtt	aaccaccatc	aaacaggatt	1650
1651	ttcgcctgct	ggggcaaacc	agcgtggacc	gcttgctgca	actctctcag	1700
1701	ggccaggcgg	tgaagggcaa	tcagctgttg	cccgtctcac	tggtgaaaag	1750
1751	aaaaaccacc	ctggcgccca	atacgcaaac	cgcctctccc	cgcgcgttgg	1800
1801	ccgattcatt	aatgcagctg	gcacgacagg	tttcccgact	ggaaagcggg	1850
1851	cagtgagcgc	aacgcaatta	atgtaagtta	gctcactcat	taggcaccgg	1900
1901	gatctcgacc	gatgcccttg	agagccttca	acccagtcag	ctccttccgg	1950
1951	tgggcgcggg	gcatgactat	cgtcgccgca	cttatgactg	tcttctttat	2000
2001	catgcaactc	gtaggacagg	tgccggcagc	gctctgggtc	attttcggcg	2050
2051	aggaccgctt	tcgctggagc	gcgacgatga	tcggcctgtc	gcttgcggta	2100
2101	ttcggaatct	tgcacgccct	cgctcaagcc	ttcgtcactg	gtcccgccac	2150
2151	caaacgtttc	ggcgagaagc	aggccattat	cgccggcatg	gcggccccac	2200
2201	gggtgcgcat	gatcgtgctc	ctgtcgttga	ggacccggct	aggctggcgg	2250
2251	ggttgcctta	ctggttagca	gaatgaatca	ccgatacgcg	agcgaacgtg	2300
2301	aagcgactgc	tgctgcaaaa	cgtctgcgac	ctgagcaaca	acatgaatgg	2350
2351	tcttcggttt	ccgtgtttcg	taaagtctgg	aaacgcggaa	gtcagcgccc	2400
2401	tgcaccatta	tgttccggat	ctgcatcgca	ggatgctgct	ggctaccctg	2450
2451	tggaacacct	acatctgtat	taacgaagcg	ctggcattga	ccctgagtga	2500
2501	tttttctctg	gtcccgccgc	atccataccg	ccagttgttt	accctcacaa	2550
2551	cgttccagta	accgggcatg	ttcatcatca	gtaacccgta	tcgtgagcat	2600
2601	cctctctcgt	ttcatcggta	tcattacccc	catgaacaga	aatccccctt	2650
2651	acacggaggc	atcagtgacc	aaacaggaaa	aaaccgccct	taacatggcc	2700
2701	cgctttatca	gaagccagac	attaacgctt	ctggagaaac	tcaacgagct	2750
2751	ggacgcggat	gaacaggcag	acatctgtga	atcgcttcac	gaccacgctg	2800
2801	atgagcttta	ccgcagctgc	ctcgcgcgtt	tcggtgatga	cggtgaaaac	2850
2851	ctctgacaca	tgcagctccc	ggagacggtc	acagcttgtc	tgtaagcgga	2900
2901	tgccgggagc	agacaagccc	gtcagggcgc	gtcagcgggt	gttggcgggt	2950
2951	gtcggggcgc	agccatgacc	cagtcacgta	gcgatagcgg	agtgtatact	3000
3001	ggcttaacta	tgcggcatca	gagcagattg	tactgagagt	gcaccatata	3050
3051	tgcggtgtga	aataccgcac	agatgcgtaa	ggagaaaata	ccgcatcagg	3100
3101	cgctcttccg	cttcctcgct	cactgactcg	ctgcgctcgg	tcgttcggct	3150
3151	gcggcgagcg	gtatcagctc	actcaaaggc	ggtaatacgg	ttatccacag	3200
3201	aatcagggga	taacgcagga	aagaacatgt	gagcaaaagg	ccagcaaaag	3250
3251	gccaggaacc	gtaaaaaggc	cgcgttgctg	gcgtttttcc	ataggctccg	3300
3301	cccccctgac	gagcatcaca	aaaatcgacg	ctcaagtcag	aggtggcgaa	3350
3351	acccgacagg	actataaaga	taccaggcgt	ttccccctgg	aagctccctc	3400
3401	gtgcgctctc	ctgttccgac	cctgccgctt	accggatacc	tgtccgcctt	3450
3451	tctcccttcg	ggaagcgtgg	cgctttctca	tagctcacgc	tgtaggtatc	3500
3501	tcagttcggt	gtaggtcgtt	cgctccaagc	tgggctgtgt	gcacgaaccc	3550
3551	cccgttcagc	ccgaccgctg	cgccttatcc	ggtaactatc	gtcttgagtc	3600
3601	caacccggta	agacacgact	tatcgccact	ggcagcagcc	actggtaaca	3650
3651	ggattagcag	agcgaggtat	gtaggcggtg	ctacagagtt	cttgaagtgg	3700
3701	tggcctaact	acggctacac	tagaaggaca	gtatttggta	tctgcgctct	3750
3751	gctgaagcca	gttaccttcg	gaaaaagagt	tggtagctct	tgatccggca	3800
3801	aacaaaccac	cgctggtagc	ggtggttttt	ttgtttgcaa	gcagcagatt	3850
3851	acgcgcagaa	aaaaaggatc	tcaagaagat	cctttgatct	tttctacggg	3900
3901	gtctgacgct	cagtggaacg	aaaactcacg	ttaagggatt	ttggtcatga	3950
3951	acaataaaac	tgtctgctta	cataaacagt	aatacaaggg	gtgttatgag	4000
4001	ccatattcaa	cgggaaacgt	cttgctctag	gccgcgatta	aattccaaca	4050
4051	tggatgctga	tttatatggg	tataaatggg	ctcgcgataa	tgtcgggcaa	4100
4101	tcaggtgcga	caatctatcg	attgtatggg	aagcccgatg	cgccagagtt	4150
4151	gtttctgaaa	catggcaaag	gtagcgttgc	caatgatgtt	acagatgaga	4200
4201	tggtcagact	aaactggctg	acggaattta	tgcctcttcc	gaccatcaag	4250
4251	cattttatcc	gtactcctga	tgatgcatgg	ttactcacca	ctgcgatccc	4300
4301	cgggaaaaca	gcattccagg	tattagaaga	atatcctgat	tcaggtgaaa	4350
4351	atattgttga	tgcgctggca	gtgttcctgc	gccggttgca	ttcgattcct	4400
4401	gtttgtaatt	gtccttttaa	cagcgatcgc	gtatttcgtc	tcgctcaggc	4450
4451	gcaatcacga	atgaataacg	gtttggttga	tgcgagtgat	tttgatgacg	4500
4501	agcgtaatgg	ctggcctgtt	gaacaagtct	ggaaagaaat	gcataaactt	4550
4551	ttgccattct	caccggattc	agtcgtcact	catggtgatt	tctcacttga	4600
4601	taaccttatt	tttgacgagg	ggaaattaat	aggttgtatt	gatgttggac	4650
4651	gagtcggaat	cgcagaccga	taccaggatc	ttgccatcct	atggaactgc	4700
4701	ctcggtgagt	tttctccttc	attacagaaa	cggctttttc	aaaaatatgg	4750
4751	tattgataat	cctgatatga	ataaattgca	gtttcatttg	atgctcgatg	4800
4801	agtttttcta	agaattaatt	catgagcgga	tacatatttg	aatgtattta	4850
4851	gaaaaataaa	caaatagggg	ttccgcgcac	atttccccga	aaagtgccac	4900
4901	ctgaaattgt	aaacgttaat	attttgttaa	aattcgcgtt	aaatttttgt	4950
4951	taaatcagct	cattttttaa	ccaataggcc	gaaatcggca	aaatccctta	5000
5001	taaatcaaaa	gaatagaccg	agatagggtt	gagtgttgtt	ccagtttgga	5050
5051	acaagagtcc	actattaaag	aacgtggact	ccaacgtcaa	agggcgaaaa	5100
5101	accgtctatc	agggcgatgg	cccactacgt	gaaccatcac	cctaatcaag	5150
5151	ttttttgggg	tcgaggtgcc	gtaaagcact	aaatcggaac	cctaaaggga	5200
5201	gcccccgatt	tagagcttga	cggggaaagc	cggcgaacgt	ggcgagaaag	5250
5251	gaagggaaga	aagcgaaagg	agcgggcgct	agggcgctgg	caagtgtagc	5300
5301	ggtcacgctg	cgcgtaacca	ccacacccgc	cgcgcttaat	gcgccgctac	5350
5351	agggcgcgtc	ccattcgcca	5370