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低温运输,-20℃保存
1年
pET-29b(+)
大量
信裕生物
名称 | 编号 | 规格 | 价格 | 手册 |
---|---|---|---|---|
pET-29b(+)(pET29b) | 60908-3426y |
质粒编号:60908-3426
质粒名称: pET-29b(+)
其他名称:
质粒大小(bp): 5370
抗性: Kanamycin (卡那霉素)
质粒类型:大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-S, N-Thrombin, C-His
5′ 测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Nterm thrombin cleavage site; a,b,c vary by MCS.
质粒图谱:
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
lacI | 217 | 1856 | |
T7_transl_en_RBS | 320 | 304 | |
lacO | 365 | 338 | |
T7_promoter | 383 | 365 | |
tet (300 – 563) | 419 | 682 | |
pBRrevBam_primer | 490 | 471 | |
tet (576 – 851) | 1915 | 2190 | |
ROP | 2665 | 2856 | |
pGEX_3_primer | 2872 | 2850 | |
pBR322_origin | 3890 | 3271 | |
KanR2 | 3996 | 4811 |
ORF | Start | End | |
---|---|---|---|
ORF frame 2 | 296 | 1024 | |
ORF frame 3 | 897 | 1856 | |
ORF frame 3 | 3996 | 4811 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
EcoRV | 206 |
NcoI | 211 |
KpnI | 237 |
BglII | 240 |
BstBI | 267 |
NdeI | 294 |
XbaI | 332 |
BclI | 1138 |
ApaI | 1335 |
HpaI | 1630 |
FspI | 2206 |
NruI | 4084 |
SmaI | 4301 |
XmaI | 4299 |
质粒序列:
1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
201 | tccgatatcg | ccatggaacc | gcgtggcacc | agggtaccca | gatctgggct | 250 |
251 | gtccatgtgc | tggcgttcga | atttagcagc | agcggtttct | ttcatatgta | 300 |
301 | tatctccttc | ttaaagttaa | acaaaattat | ttctagaggg | gaattgttat | 350 |
351 | ccgctcacaa | ttcccctata | gtgagtcgta | ttaatttcgc | gggatcgaga | 400 |
401 | tcgatctcga | tcctctacgc | cggacgcatc | gtggccggca | tcaccggcgc | 450 |
451 | cacaggtgcg | gttgctggcg | cctatatcgc | cgacatcacc | gatggggaag | 500 |
501 | atcgggctcg | ccacttcggg | ctcatgagcg | cttgtttcgg | cgtgggtatg | 550 |
551 | gtggcaggcc | ccgtggccgg | gggactgttg | ggcgccatct | ccttgcatgc | 600 |
601 | accattcctt | gcggcggcgg | tgctcaacgg | cctcaaccta | ctactgggct | 650 |
651 | gcttcctaat | gcaggagtcg | cataagggag | agcgtcgaga | tcccggacac | 700 |
701 | catcgaatgg | cgcaaaacct | ttcgcggtat | ggcatgatag | cgcccggaag | 750 |
751 | agagtcaatt | cagggtggtg | aatgtgaaac | cagtaacgtt | atacgatgtc | 800 |
801 | gcagagtatg | ccggtgtctc | ttatcagacc | gtttcccgcg | tggtgaacca | 850 |
851 | ggccagccac | gtttctgcga | aaacgcggga | aaaagtggaa | gcggcgatgg | 900 |
901 | cggagctgaa | ttacattccc | aaccgcgtgg | cacaacaact | ggcgggcaaa | 950 |
951 | cagtcgttgc | tgattggcgt | tgccacctcc | agtctggccc | tgcacgcgcc | 1000 |
1001 | gtcgcaaatt | gtcgcggcga | ttaaatctcg | cgccgatcaa | ctgggtgcca | 1050 |
1051 | gcgtggtggt | gtcgatggta | gaacgaagcg | gcgtcgaagc | ctgtaaagcg | 1100 |
1101 | gcggtgcaca | atcttctcgc | gcaacgcgtc | agtgggctga | tcattaacta | 1150 |
1151 | tccgctggat | gaccaggatg | ccattgctgt | ggaagctgcc | tgcactaatg | 1200 |
1201 | ttccggcgtt | atttcttgat | gtctctgacc | agacacccat | caacagtatt | 1250 |
1251 | attttctccc | atgaagacgg | tacgcgactg | ggcgtggagc | atctggtcgc | 1300 |
1301 | attgggtcac | cagcaaatcg | cgctgttagc | gggcccatta | agttctgtct | 1350 |
1351 | cggcgcgtct | gcgtctggct | ggctggcata | aatatctcac | tcgcaatcaa | 1400 |
1401 | attcagccga | tagcggaacg | ggaaggcgac | tggagtgcca | tgtccggttt | 1450 |
1451 | tcaacaaacc | atgcaaatgc | tgaatgaggg | catcgttccc | actgcgatgc | 1500 |
1501 | tggttgccaa | cgatcagatg | gcgctgggcg | caatgcgcgc | cattaccgag | 1550 |
1551 | tccgggctgc | gcgttggtgc | ggacatctcg | gtagtgggat | acgacgatac | 1600 |
1601 | cgaagacagc | tcatgttata | tcccgccgtt | aaccaccatc | aaacaggatt | 1650 |
1651 | ttcgcctgct | ggggcaaacc | agcgtggacc | gcttgctgca | actctctcag | 1700 |
1701 | ggccaggcgg | tgaagggcaa | tcagctgttg | cccgtctcac | tggtgaaaag | 1750 |
1751 | aaaaaccacc | ctggcgccca | atacgcaaac | cgcctctccc | cgcgcgttgg | 1800 |
1801 | ccgattcatt | aatgcagctg | gcacgacagg | tttcccgact | ggaaagcggg | 1850 |
1851 | cagtgagcgc | aacgcaatta | atgtaagtta | gctcactcat | taggcaccgg | 1900 |
1901 | gatctcgacc | gatgcccttg | agagccttca | acccagtcag | ctccttccgg | 1950 |
1951 | tgggcgcggg | gcatgactat | cgtcgccgca | cttatgactg | tcttctttat | 2000 |
2001 | catgcaactc | gtaggacagg | tgccggcagc | gctctgggtc | attttcggcg | 2050 |
2051 | aggaccgctt | tcgctggagc | gcgacgatga | tcggcctgtc | gcttgcggta | 2100 |
2101 | ttcggaatct | tgcacgccct | cgctcaagcc | ttcgtcactg | gtcccgccac | 2150 |
2151 | caaacgtttc | ggcgagaagc | aggccattat | cgccggcatg | gcggccccac | 2200 |
2201 | gggtgcgcat | gatcgtgctc | ctgtcgttga | ggacccggct | aggctggcgg | 2250 |
2251 | ggttgcctta | ctggttagca | gaatgaatca | ccgatacgcg | agcgaacgtg | 2300 |
2301 | aagcgactgc | tgctgcaaaa | cgtctgcgac | ctgagcaaca | acatgaatgg | 2350 |
2351 | tcttcggttt | ccgtgtttcg | taaagtctgg | aaacgcggaa | gtcagcgccc | 2400 |
2401 | tgcaccatta | tgttccggat | ctgcatcgca | ggatgctgct | ggctaccctg | 2450 |
2451 | tggaacacct | acatctgtat | taacgaagcg | ctggcattga | ccctgagtga | 2500 |
2501 | tttttctctg | gtcccgccgc | atccataccg | ccagttgttt | accctcacaa | 2550 |
2551 | cgttccagta | accgggcatg | ttcatcatca | gtaacccgta | tcgtgagcat | 2600 |
2601 | cctctctcgt | ttcatcggta | tcattacccc | catgaacaga | aatccccctt | 2650 |
2651 | acacggaggc | atcagtgacc | aaacaggaaa | aaaccgccct | taacatggcc | 2700 |
2701 | cgctttatca | gaagccagac | attaacgctt | ctggagaaac | tcaacgagct | 2750 |
2751 | ggacgcggat | gaacaggcag | acatctgtga | atcgcttcac | gaccacgctg | 2800 |
2801 | atgagcttta | ccgcagctgc | ctcgcgcgtt | tcggtgatga | cggtgaaaac | 2850 |
2851 | ctctgacaca | tgcagctccc | ggagacggtc | acagcttgtc | tgtaagcgga | 2900 |
2901 | tgccgggagc | agacaagccc | gtcagggcgc | gtcagcgggt | gttggcgggt | 2950 |
2951 | gtcggggcgc | agccatgacc | cagtcacgta | gcgatagcgg | agtgtatact | 3000 |
3001 | ggcttaacta | tgcggcatca | gagcagattg | tactgagagt | gcaccatata | 3050 |
3051 | tgcggtgtga | aataccgcac | agatgcgtaa | ggagaaaata | ccgcatcagg | 3100 |
3101 | cgctcttccg | cttcctcgct | cactgactcg | ctgcgctcgg | tcgttcggct | 3150 |
3151 | gcggcgagcg | gtatcagctc | actcaaaggc | ggtaatacgg | ttatccacag | 3200 |
3201 | aatcagggga | taacgcagga | aagaacatgt | gagcaaaagg | ccagcaaaag | 3250 |
3251 | gccaggaacc | gtaaaaaggc | cgcgttgctg | gcgtttttcc | ataggctccg | 3300 |
3301 | cccccctgac | gagcatcaca | aaaatcgacg | ctcaagtcag | aggtggcgaa | 3350 |
3351 | acccgacagg | actataaaga | taccaggcgt | ttccccctgg | aagctccctc | 3400 |
3401 | gtgcgctctc | ctgttccgac | cctgccgctt | accggatacc | tgtccgcctt | 3450 |
3451 | tctcccttcg | ggaagcgtgg | cgctttctca | tagctcacgc | tgtaggtatc | 3500 |
3501 | tcagttcggt | gtaggtcgtt | cgctccaagc | tgggctgtgt | gcacgaaccc | 3550 |
3551 | cccgttcagc | ccgaccgctg | cgccttatcc | ggtaactatc | gtcttgagtc | 3600 |
3601 | caacccggta | agacacgact | tatcgccact | ggcagcagcc | actggtaaca | 3650 |
3651 | ggattagcag | agcgaggtat | gtaggcggtg | ctacagagtt | cttgaagtgg | 3700 |
3701 | tggcctaact | acggctacac | tagaaggaca | gtatttggta | tctgcgctct | 3750 |
3751 | gctgaagcca | gttaccttcg | gaaaaagagt | tggtagctct | tgatccggca | 3800 |
3801 | aacaaaccac | cgctggtagc | ggtggttttt | ttgtttgcaa | gcagcagatt | 3850 |
3851 | acgcgcagaa | aaaaaggatc | tcaagaagat | cctttgatct | tttctacggg | 3900 |
3901 | gtctgacgct | cagtggaacg | aaaactcacg | ttaagggatt | ttggtcatga | 3950 |
3951 | acaataaaac | tgtctgctta | cataaacagt | aatacaaggg | gtgttatgag | 4000 |
4001 | ccatattcaa | cgggaaacgt | cttgctctag | gccgcgatta | aattccaaca | 4050 |
4051 | tggatgctga | tttatatggg | tataaatggg | ctcgcgataa | tgtcgggcaa | 4100 |
4101 | tcaggtgcga | caatctatcg | attgtatggg | aagcccgatg | cgccagagtt | 4150 |
4151 | gtttctgaaa | catggcaaag | gtagcgttgc | caatgatgtt | acagatgaga | 4200 |
4201 | tggtcagact | aaactggctg | acggaattta | tgcctcttcc | gaccatcaag | 4250 |
4251 | cattttatcc | gtactcctga | tgatgcatgg | ttactcacca | ctgcgatccc | 4300 |
4301 | cgggaaaaca | gcattccagg | tattagaaga | atatcctgat | tcaggtgaaa | 4350 |
4351 | atattgttga | tgcgctggca | gtgttcctgc | gccggttgca | ttcgattcct | 4400 |
4401 | gtttgtaatt | gtccttttaa | cagcgatcgc | gtatttcgtc | tcgctcaggc | 4450 |
4451 | gcaatcacga | atgaataacg | gtttggttga | tgcgagtgat | tttgatgacg | 4500 |
4501 | agcgtaatgg | ctggcctgtt | gaacaagtct | ggaaagaaat | gcataaactt | 4550 |
4551 | ttgccattct | caccggattc | agtcgtcact | catggtgatt | tctcacttga | 4600 |
4601 | taaccttatt | tttgacgagg | ggaaattaat | aggttgtatt | gatgttggac | 4650 |
4651 | gagtcggaat | cgcagaccga | taccaggatc | ttgccatcct | atggaactgc | 4700 |
4701 | ctcggtgagt | tttctccttc | attacagaaa | cggctttttc | aaaaatatgg | 4750 |
4751 | tattgataat | cctgatatga | ataaattgca | gtttcatttg | atgctcgatg | 4800 |
4801 | agtttttcta | agaattaatt | catgagcgga | tacatatttg | aatgtattta | 4850 |
4851 | gaaaaataaa | caaatagggg | ttccgcgcac | atttccccga | aaagtgccac | 4900 |
4901 | ctgaaattgt | aaacgttaat | attttgttaa | aattcgcgtt | aaatttttgt | 4950 |
4951 | taaatcagct | cattttttaa | ccaataggcc | gaaatcggca | aaatccctta | 5000 |
5001 | taaatcaaaa | gaatagaccg | agatagggtt | gagtgttgtt | ccagtttgga | 5050 |
5051 | acaagagtcc | actattaaag | aacgtggact | ccaacgtcaa | agggcgaaaa | 5100 |
5101 | accgtctatc | agggcgatgg | cccactacgt | gaaccatcac | cctaatcaag | 5150 |
5151 | ttttttgggg | tcgaggtgcc | gtaaagcact | aaatcggaac | cctaaaggga | 5200 |
5201 | gcccccgatt | tagagcttga | cggggaaagc | cggcgaacgt | ggcgagaaag | 5250 |
5251 | gaagggaaga | aagcgaaagg | agcgggcgct | agggcgctgg | caagtgtagc | 5300 |
5301 | ggtcacgctg | cgcgtaacca | ccacacccgc | cgcgcttaat | gcgccgctac | 5350 |
5351 | agggcgcgtc | ccattcgcca | 5370 |
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